Rad51L1 (RAD51B) Rabbit Polyclonal Antibody

CAT#: AP20559PU-N

Rad51L1 (RAD51B) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Rad51B antibody (Cat.-No.: AP20559PU-N) in extracts from COS-7 cells.

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  Immunohistochemistry (IHC) analyzes of Rad51B antibody (Cat.-No.: AP20559PU-N) in paraffin-embedded human pancreas tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human Rad51B.
• Specificity: This antibody detects endogenous levels of Rad51B protein
  (region surrounding Gly226).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 45 kDa
• Gene Name: RAD51 paralog B
• Database Link:
  Entrez Gene 19363 MouseEntrez Gene 5890 Human
  UniProt ID O15315
• Background: The DNA repair proteins Rad50, Rad51A-D, Rad52 and Rad54 A and B are key components of the double-strand break repair pathway (DSBR). The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair. Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3. Rad51B is a widely expressed protein that is involved in the repair of double-stranded DNA breaks that can arise during DNA replication. It can form a dimer with Rad51C and this complex shows single-stranded DNAdependent ATPase activity.
• Synonyms: R51H2, RAD51B, RAD51 homolog B, RAD51-like protein 1, REC2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination