ISG15 (N-term) Rabbit Polyclonal Antibody

CAT#: AP11162PU-N

ISG15 (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-ISG15 Pab to detect ISG15 in mouse kidney tissue lysate.

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  Western blot analysis of ISG15 (arrow) using Rabbit polyclonal hISG15-A46. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (Lane 2)

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  Western blot analysis in 293 cell line lysates (35ug/lane) using ISG15 Antibody (A46). This demonstrates the ISG15 antibody detected the ISG15 protein (arrow).

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  Western blot analysis using ISG15 Antibody in Jurkat, K562 and AML-2 cell line lysates treated with a small molecular of 0, 10, 20, 30uM for 24 hours. Offered friendly by Biyin Cao, Soochow University, Center for Blood Research.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Western blot: 1/1,000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 31~61 amino acids from the N-terminal region of Human ISG15.
• Specificity: This antibody is specific to Human ISG15 (N-term).
  Other species not tested.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ISG15 ubiquitin-like modifier
• Database Link:
  Entrez Gene 9636 Human
  UniProt ID P05161
• Background: ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.
• Synonyms: UCRP