RANKL (TNFSF11) Rabbit Polyclonal Antibody

CAT#: AP06633PU-N

RANKL (TNFSF11) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of RANKL antibody in extracts from HUVEC cells.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 51-100 of Human RANKL.
• Specificity: This antibody detects endogenous levels of RANKL protein. (region surrounding Ala72)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% (by SDS-PAGE))
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 40 kDa
• Gene Name: tumor necrosis factor superfamily member 11
• Database Link:
  Entrez Gene 8600 Human
  UniProt ID O14788
• Background: Members of the tumor necrosis factor (TNF) receptor superfamily interact with signaling molecules of the TNF receptor-associated factor (TRAF) family to activate the NFκB and JNK pathways. RANK (receptor activator of NFkappaB) is a member of the TNFR family identified on dendritic cells. This type I membrane receptor is expressed in a broad range of tissues. The C-terminus of RANK is required for RANK to bind TRAF2, 5 and 6, and it is also necessary for stimulating NFκB activation. The ligand for this receptor, RANKL (also designated TRANCE, OPGL or ODF), is a type II transmembrane protein expressed primarily in lymphoid tissues and T cell lines. RANKL appears to be an important regulator of T cells and osteoclasts.
• Synonyms: OPGL, RANK Ligand, RANKL, TRANCE, TNFSF11, ODF