BCL2 Rabbit Polyclonal Antibody

CAT#: AP06028PU-N

BCL2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Bcl-2 antibody at 1/500 dilution Lane 1:MCF-7 cell lysate Lane 2:NIH-3T3 cell lysate Lane 3:Raw264.7 cell lysate

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  Immunohistochemistry analysis of Bcl-2 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 31-80 of Human Bcl-2.
• Specificity: This antibody detects endogenous levels of BCL-2 protein.
  (region surrounding Asp64)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~26 kDa
• Gene Name: B-cell CLL/lymphoma 2
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Background: Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane.
• Synonyms: BCL2, Bcl-2 alpha