ATF 4 (ATF4) Rabbit Polyclonal Antibody

CAT#: AP06024PU-N

ATF 4 (ATF4) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ATF4 antibody at 1/500 dilution Lane 1:MCF-7 cell lysate Lane 2:Mouse brain tissue lysate Lane 3:Rat brain tissue lysate

• 

  Immunohistochemistry (IHC) analysis of ATF4 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human ATF4.
• Specificity: This antibody detects endogenous levels of ATF4 protein (region surrounding Arg239).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 42 kDa
• Gene Name: activating transcription factor 4
• Database Link:
  Entrez Gene 468 Human
  UniProt ID P18848
• Background: ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).
• Synonyms: CREB2, CREB-2, TXREB, Activating transcription factor 4, ATF-4