PCNA Mouse Monoclonal Antibody [Clone ID: SPM350]

CAT#: AM50151PU-S

PCNA mouse monoclonal antibody, clone SPM350, Purified

Product Images

Formalin-paraffin human tonsil stained with PCNA Antibody (Clone SPM350)

Specifications

Product Data

• Clone Name: SPM350
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.25-0.5 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Tonsil or reactive lymph node.
• Reactivities: Chicken, Drosophila, Human, Mouse, Rat, Yeast, Zebrafish
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Rat PCNA/Protein A fusion protein.
• Specificity: Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
  This MAb is excellent for multiple applications.
  Cellular Localization: Predominantly nuclear, some cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 36 kDa
• Gene Name: proliferating cell nuclear antigen
• Database Link:
  Entrez Gene 5111 Human
  UniProt ID P12004
• Synonyms: Cyclin