GFAP Mouse Monoclonal Antibody [Clone ID: SPM507]
Other products for "GFAP"
Specifications
Product Data | |
Clone Name | SPM507 |
Applications | FC, IF, IHC, IP, WB |
Recommended Dilution | Flow Cytometry: 0.5 µg/106 cells in 0.1 ml. Immunofluorescence: 1-2 µg/ml. Western Blot: 0.5-1 µg/ml. Immunoprecipitation: 1-2 μg/500 µg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Brain or Astrocytoma. |
Reactivities | Bovine, Chicken, Human, Mouse, Porcine, Rabbit |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | GFAP isolated from pig spinal cord. |
Specificity | This Monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Cellular Localization: Cytoplasmic. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
Predicted Protein Size | ~50 kDa |
Gene Name | glial fibrillary acidic protein |
Database Link | |
Background | Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system. |
Synonyms | Glial Fibrillary Acidic Protein |
Reference Data |
Documents
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