Mitochondria Mouse Monoclonal Antibody [Clone ID: 113-1]

CAT#: AM31831PU-N

Mitochondria mouse monoclonal antibody, clone 113-1, Ig Fraction

Product Images

Western blot of HeLa lysate showing specific immunolabeling of the ~ 60k mitochondrial protein.

Specifications

Product Data

• Clone Name: 113-1
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/1,000.
  Immunofluorescence: 1/800.
  Immunohistochemistry: 1/800.
  The antibody works on Acetone fixed and 2% paraformaldehyde fixed cells.
  The antibody also works for Immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) Cryostat sections.
  Successful use in Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded was reported by some researchers.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human cell homogenate
• Specificity: Specific for Human Mitochondria in all cell types.
  This antibody is an excellent marker for Human cells in xenographic model research.
  It reacts specifically with Human cells, including neurons and embryonic stem cells.
  It does not react with Mouse or Rat mitochondria.
• Formulation: State: Ig Fraction
  State: Liquid Total IgG fraction
• Purification: Immunoglobulin Fractionation
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Background: Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998).
• Synonyms: Mitochondrial Marker