CD8A Mouse Monoclonal Antibody [Clone ID: CC63]

CAT#: AM05901PU-S

CD8A mouse monoclonal antibody, clone CC63, Purified

Product Images

Bovine lymph node stained with Mouse anti Bovine CD8 Antibody Cat.-No AM05901PU-S (Clone CC63). 10x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK

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  Bovine lymph node stained with Mouse anti Bovine CD8 Antibody Cat.-No AM05901PU-S (Clone CC63). 40x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK

Specifications

Product Data

• Clone Name: CC63
• Applications: FC, IF, IHC, IP
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections.
  Immunoprecipitation.
  Immunofluorescence/Immunocytochemistry.
  Flow Cytometry: Use 10 µl of 1/50 diluted CD8 antibody to label 10⁶ cells in 100 µl.
• Reactivities: Bovine, Goat, Sheep
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody reacts with the Bovine CD8 antigen expressed by a subset of T lymphocytes.
  The antibody precipitates molecules of 34kD and 38kD under reducing conditions.
  Clone CC63 has been reported as being suitable for use on Formalin Dichromate (FD5) Fixed Paraffin Embedded tissue with amplification and antigen retrieval techniques (See Gutierrez, M. et al. for details).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 281060 Bovine
  UniProt ID P31783
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL