Lentivirus is a powerful gene delivery tool. Lentivirus can transduce most cells, including easy-to-transfect cells, hard-to-transfect cells, dividing, non-dividing and primary cells. The transduction efficiency can easily reach to 100% for most common cell lines.
What is lentivirus and how to make lentivirus? OriGene has a very informative lentivirus 101 ebook Which can quickly transform you into a lentivirus expert.
Lentiviral transfer vectors (lenti vectors containing your gene) need essential viral genes to package into lentiviral particles. Lentiviral packaging genes are provided on separate plasmids – lentiviral packaging plasmids. OriGene’s Lenti-vpak packaging kit contains all necessary lentiviral production genes and is designed to package the third generation lentivectors into high titer viral particles, If you have your packaging plasmids, OriGene has a specialized lentivirus production transfection reagent, LentiTran, which is specifically formulated for high lentivirus production.
Optimized for high packaging efficiency: Premix of the packaging plasmids at optimal ratio
A potent transfection reagent included
Simple to use: Transfect and harvest viral supernatant
HT1080 cells transduced with GFP lentiviral particles produced from Lenti-vpak packaging kit
For Regular Lentiviral Particle Packaging
Lentivirus produced will integrate into the host genome, enabling stable cell selection
|TR30037||Lenti-vpak packaging kit - packaging plasmids and transfection reagent||10 rxns||In Stock|
|TR30037P5||Lenti-vpak packaging kit - packaging plasmids and transfection reagent, 5 pack of TR30037||5 x 10 rxns||In Stock|
TR30022 has been upgraded to TR30037.
For integration-deficient Lentiviral Particle (IDLV) Packaging
|TR30036||IDLV Lenti packaging kit, integration deficient- packaging plasmids and transfection reagent||10 rxns||In Stock|
|TR30036P5||IDLV Lenti packaging kit, integration deficient- packaging plasmids and transfection reagent, 5 pack of TR30036||5 x 10 rxns||In Stock|
Does the viral titer need to be measured?
Virus titration will show how successful your lentivirus production is and will help to trouble shoot if viral transduction fails. Therefore virus titration is necessary. In addition, if your cells are hard to transduce and optimization is needed by using different MOI (Multiplicity Of Infection), the lentiviral titer needs to be measured.
What is MOI and how to calculate the amount of virus needed?
MOI is the number of viral particles per cell. (Recommended lentivirus MOI for common cell lines)
- How to calculate the total number of viral particles for certain MOI?
- How to calculate the amount of virus needed per well?
If you want to use MOI of 10 and there are 10^4 cells in the well/dish, what is the total number of viral particles that are needed? The total viral particles is MOI x number of cells, 10 x 10^4 = 10^5 particles.
If the lentiviral titer is 10^7 TU/mL, the amount of virus needed is 10^5/10^7 = 0.01mL
One-Wash™ Lentivirus Titer Kit
|TR30038||Lenti ELISA Kit||One-Wash™ Lentivirus Titer Kit, HIV-1 p24 ELISA|
|TR30038P5||Lenti ELISA Kit||One-Wash™ Lentivirus Titer Kit, HIV-1 p24 ELISA, 5 pack of TR30038|
- Simplified Workflow
- No need for 37°C incubation
- Shortened protocol
- One wash instead of 4 washes
- Sample binding: 2 hrs vs overnight
If the lentiviral titer is low, the virus can be concentrated quickly with Lentiviral Concentrator, easily reaching to 10^9 TU/mL. If higher titer is needed or ultra pure virus is needed for in vivo animal studies, ultra centrifugation is needed. Custom lentivirus Production of flexible titers and volumes are provided from OriGene.
Lentiviral Concentrator Features
- Rescue low-titer viral particles, up to 100 fold
- Simple mix and centrifuge, no need for ultracentrifugation
- Easily scaled up for large volumes
- Protection from freeze-thaw after concentration
- Download Datasheet
|TR30025||Lentivirus concentration solution (5X), 50ml||In Stock|
|TR30026||Lentivirus concentration solution (5X), 4x50ml||In Stock|
lentiviral GFP particles (8 ml) were concentrated using TR30025, resuspended in 80ul of PBS.
GFP fluorescence image 72 hrs post transduction of HT1080 cells using lenti particles before and after concentration.
Lentiviral particles are enveloped viruses and tend to lose infectivity during storage, even at -80°C. OriGene developed a lentiviral storage buffer, Lentivirus Stabilizer, which can preserve the infectivity for at least one year at - 80°C.
|TR30039||Lentivirus stabilizer, preserving lentiviral infectivity, 5 mL|
|TR30039L||Lentivirus stabilizer, preserving the lentiviral infectivity, 20 mL|
- Stabilizing lentivirus in storage
- Prolong storage, > 1 year
- Protect freeze and thaws: When used together with Lenti concentrator (TR30025)
- Download datasheet
Lentivirus Stabilizer Well Preserved the Infectivity of Lentivirus
Lenti-GFP particles (cat# PS100071V) freshly made (left panel). The viruses in culture media or Lenti Stabilizer were incubated at 37°C for 5 days. HEK293T cells were transduced with the above lentiviruses. Fluorescent images were taken 48 hrs post transduction.
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