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KRT20 HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T

Cytokeratin 20 (1-424, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

Cytokeratin 20 (1-424, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

KRT20 CRISPRa kit - CRISPR gene activation of human keratin 20

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

qSTAR qPCR primer pairs against Homo sapiens gene KRT20

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

KRT20 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Cytokeratin 20 human recombinant protein, 0.25 mg

Expression Host E. coli

Cytokeratin 20 human recombinant protein, 0.1 mg

Expression Host E. coli

qPCR primer pairs and template standards against Homo sapiens gene KRT20

Application Plasmid of exact quantity for transcript copy number calculation

3' UTR clone of keratin 20 (KRT20) for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

KRT20 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

KRT20 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin

Transient overexpression of KRT20 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

Other Names CD20; CK-20; CK20; K20; KRT21
Accession Number NM_019010, NP_061883