Traumatic Brain Injury Research Tools

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C17orf97 (Myc-DDK-tagged)-Human chromosome 17 open reading frame 97 (C17orf97). Note: ORF is codon optimized

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

C17orf97 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro

C17orf97 (tGFP-tagged) - Human chromosome 17 open reading frame 97 (C17orf97). Note: ORF is codon optimized

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

C17orf97 CRISPRa kit - CRISPR gene activation of human chromosome 17 open reading frame 97

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

qSTAR qPCR primer pairs against Homo sapiens gene C17orf97

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

3' UTR clone of chromosome 17 open reading frame 97 (C17orf97) for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

C17orf97 (untagged)-Human chromosome 17 open reading frame 97 (C17orf97)

Vector pCMV6 series
Tag Tag Free

C17orf97 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

C17orf97 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

C17orf97 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

C17orf97 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin