Secondary Antibodies

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Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252BT-N

USD 865.00

2 Weeks

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Biotin

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
General Recommended Dilutions:
Immunocytochemistry.
Immunohistochemistry (Paraffin).
Histochemical Use: 1/10-1/40.
ELISA: 1/100-1/2000. 
Western blot: 1/200-1/4000.
Reactivities Human
Conjugation Biotin
IGHG4 Mouse Monoclonal Antibody [Clone ID: HP6025]
SM2069AP

USD 425.00

5 Days

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections:  reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry: reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP
Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]
AM20249AF-N

USD 580.00

2 Weeks

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252HR-N

USD 865.00

2 Weeks

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
ELISA: 1/20-1/500.
Western Blot: 1/40-1/1000. 
Histochemical Use: 1/5-1/20.
Reactivities Human
Conjugation HRP
Monkey IgG (Fc specific) Goat Polyclonal Antibody
AP31438FC-N

USD 540.00

2 Weeks

Monkey IgG (Fc specific) goat polyclonal antibody, FITC

Applications Can be used:
• In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates.
• To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilution: 1/20-1/80.
Reactivities Monkey
Conjugation FITC
Rat IgA (heavy chain) Mouse Monoclonal Antibody [Clone ID: MARA-1]
SM1027P

USD 575.00

5 Days

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified

Applications ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Rat
Conjugation Unconjugated
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253F

USD 295.00

3 Days

Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for ELISA, Immunofluorescence Microscopy.
Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Reactivities Mouse
Conjugation FITC
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482HR-N

USD 705.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
depending on the method used.
Reactivities Mouse
Conjugation HRP
Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP21490BT-N

USD 705.00

2 Weeks

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000 - 1/20000.
Reactivities Rabbit
Conjugation Biotin
Rabbit IgG (H+L chain) Goat Polyclonal Antibody
R1364F

USD 295.00

3 Days

Rabbit IgG (H+L chain) goat polyclonal antibody, FITC, Lyophilized

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluoresence: 1/1,000-1/5,000.
Fluorescent Western Blot.
Reactivities Rabbit
Conjugation FITC
Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612F

USD 330.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunofluorescence: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation FITC
Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]
AM20255HR-N

USD 865.00

2 Weeks

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), HRP

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation HRP
Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP21490HR-N

USD 705.00

2 Weeks

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Immunocytochemistry.
Immunohistochemistry.
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Reactivities Rabbit
Conjugation HRP
Rabbit IgG (H+L chain) Goat Polyclonal Antibody
R1364TR

USD 310.00

3 Days

Rabbit IgG (H+L chain) goat polyclonal antibody, Texas Red, Lyophilized

Applications Suitable for immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1000 - 1/5000.
Reactivities Rabbit
Conjugation Texas Red
Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258AF-N

USD 580.00

2 Weeks

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated