Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody

CAT#: AP21490HR-N

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP, WB
• Recommended Dilution: ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
  Immunocytochemistry.
  Immunohistochemistry.
  Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
  Dot blot.
  Western blot.
  General Recommended Dilutions:
  Histochemical and Cytochemical Use: 1/100-1/500.
  ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
  Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
• Reactivities: Rabbit
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified immunoglobulin fractions isolated from pooled rabbit serum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes. It does not react with any non-Ig protein in rabbit serum, as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
  Cross-Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: HRP
  State: Lyophilized purified IgG fraction
  Label: Horseradish Peroxidase
  Enzyme marker: Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).
  Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life
  Molar radio: Peroxidase/IgG: ~1.7
• Reconstitution Method: Restore by adding 1.0 ml sterile distilled water.
• Concentration: appr. 10.0 mg/ml
• Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt-Precipitation and purifaction of the IgG fraction by DEAE Chromatography
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.