TNF alpha (TNF) Human siRNA Oligo Duplex (Locus ID 7124)
TNF (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
|Quality Control||Tested by ESI-MS|
|Sequences||Available with shipment|
|Stability||One year from date of shipment when stored at -20°C.|
|Number of Transfections||Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).|
|Note||Single siRNA duplex (10nmol) can be ordered.|
|Synonyms||DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F|
|Components||TNF (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 7124)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml|
|Summary||This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]|
|Performance Guranteed||OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.
For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
|The use of this RNAi has been cited in the following citations:|
Transmembrane TNF-a Facilitates HIV-1 Infection of Podocytes Cultured from Children with HIV-Associated Nephropathy
,Li, J;Das, J;Tang, P;Han, Z;Jaiswal, J;Ray, P;,
J. Am. Soc. Nephrol 2016
,PubMed ID 27811066
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.