HDAC5 Human shRNA Plasmid Kit (Locus ID 10014)

CAT#: TG312492

HDAC5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector

custom shRNA service

USD 695.00


Availability*
2 Weeks

Size
    • 5 ug/vial

Product images

Specifications

Product Data
Product Name HDAC5 Human shRNA Plasmid Kit (Locus ID 10014)
Locus ID 10014
Synonyms HD5, NY-CO-9, KIAA0600;FLJ90614; HD5; NY-CO-9; antigen NY-CO-9; histone deacetylase 5
Vector pGFP-V-RS
Antibiotic Selection Kanamycin
Format Retroviral plasmids
Kit Components HDAC5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 10014). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_001015053, NM_005474, NM_139205, XM_005256905, XM_005256906, XM_011524149, XM_011524150, XM_017023988, XM_017023989, XM_017023990, XM_017023991, XM_017023992, XM_017023993, XM_017023994, XM_017023995, XM_017023996
Summary Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations