CTLA4 Human Recombinant Protein

CAT#: TP724014

Human CTLA-4 Protein, hFc tag

Product Images

Figure 1. Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition.

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  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA-4, hFc tagged protein (TP724014) can bind Anti-CTLA4 Neutralizing antibody（BME100022） in a linear range of 0.64-80.0 ng/ml.

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  Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein (TP724014) 1µg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  CTLA-4（Gln36-Ser161）+hFc（Glu99-Ala330）
• Tag: C-Human Fc
• Predicted MW: 39.6 kDa
• Purity: > 95%
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
• Reconstitution: Reconstitute with deionized water
• Preparation: Affinity purification
• Applications: Flow Cyt
• Storage: Store the lyophilized protein at -20°C.
  After reconstitution, store the protein at -80°C for 12 months.
  Avoid repeated freezing and thawing.
• Stability: 12 months from date of despatch

Reference Data

• Locus ID: 1493
• UniProt ID: P16410
• Summary: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.