Tnfsf11 (NM_057149) Rat Recombinant Protein
Purified recombinant protein of Rat tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11).
|Description||Purified recombinant protein of Rat tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11).|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||PAMMEGSWLDVARRGKPEAQPFAHLTINAADIPSGSHKVSLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPADYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISVQVSNPSLLDPDQDATYFGAFKVQDID|
|Predicted MW||19.4 kDa|
|Purity||>95% as determined by SDS-PAGE and Coomassie blue staining|
|Buffer||Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2|
|Bioactivity||Determined by its ability to induce NFkB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml.
Cell culture factor (PMID: 30022569)
|Endotoxin||Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)|
|Storage||Store at -80°C.|
|Stability||Stable for at least 6 months from date of receipt under proper storage and handling conditions.|
|Summary||induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006]|