TSSK2 (TSSK2) (NM_053006) Human Recombinant Protein

CAT#: TP307232

Recombinant protein of human testis-specific serine kinase 2 (TSSK2)

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 Product Datasheet for 'TP307232'

USD 680.00

In Stock

    • 20 ug

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Product Data
Description Recombinant protein of human testis-specific serine kinase 2 (TSSK2)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC207232. Click on the TrueORF clone link to view cDNA and protein sequences.
Predicted MW 40.8 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Bioactivity TSSK2 activity verified in a biochemical assay: TSSK2 (testis-specific serine kinase 2) (TP307232) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. TSSK2 is a serine/threonine kinase that is highly expressed in testis and may be involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. Varying concentrations of TSSK2 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Reference Data
RefSeq NP_443732
Locus ID 23617
Refseq Size 1363
Cytogenetics 22q11.21
Refseq ORF 1074
Synonyms DGS-G; SPOGA2; STK22B; TSK2
Summary TSSK2 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]).[supplied by OMIM, Mar 2008].
Protein Families Protein Kinase, Druggable Genome
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