Thrombopoietin (22-353, His-tag) Human Protein
Thrombopoietin (22-353, His-tag) human recombinant protein, 50 µg
|Description||Thrombopoietin (22-353, His-tag) human recombinant protein, 50 µg|
|Expression cDNA Clone or AA Sequence||SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EGLEHHHHHH|
|Predicted MW||36.5 kDa|
|Concentration||0.25 mg/ml (determined by Bradford assay)|
|Purity||>85% by SDS – PAGE.|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: Phosphate buffered saline (pH 7.4) containing 10% glycerol.
|Endotoxin||< 1.0 Eu per 1 microgram of protein (determined by LAL method)|
|Preparation||Liquid purified protein|
|Protein Description||Recombinant human THPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.|
|Storage||Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||MGDF; MKCSF; ML; MPLLG; THCYT1; TPO|
|Summary||Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]|
|Protein Families||Druggable Genome, Secreted Protein|
|Protein Pathways||Hematopoietic cell lineage|