DVL1(DVL1) (NM_004421) Human Over-expression Lysate

CAT#: LC401404

DVL1 HEK293T cell transient overexpression lysate (as WB positive control)

Bulk Request/Custom Protein Development


 Product Datasheet for 'LC401404'

USD 55.00


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Size
    • 20 ug

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Specifications

Product Data
Description DVL1 HEK293T cell transient overexpression lysate (as WB positive control)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217691
Tag C-MYC/DDK
Detection Antibodies Anti-DDK Antibody (TA50011-100)
Accession Number NM_004421, NP_004412
Other Names DVL1, DRS2; DVL; DVL1L1; DVL1P1
Components 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reference Data
RefSeq NP_004412
Locus ID 1855
Cytogenetics DRS2; DVL; DVL1L1; DVL1P1
Protein Families ES Cell Differentiation/IPS, Druggable Genome
Protein Pathways Wnt signaling pathway, Notch signaling pathway, Melanogenesis, Pathways in cancer, Colorectal cancer, Basal cell carcinoma
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.