NIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell Lysate

CAT#: LY500071

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NIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell Lysate



USD 176.00


Availability*
5 Days

Size
    • 100 ug


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Specifications

Product Data
Tissue Embryo
Host Mouse
Lysis Buffer Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4)
Components 1 vial of 100ug whole cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage Shipped on dry ice. Store the lysate at -80°C and avoid repeated freeze / thaw cycles.
The lysate is stable for at least one year from date of shipping when stored at -80°C. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
Recommended Application IP, WB
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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