DNMT3B Human Gene Knockout Kit (CRISPR)

CAT#: KN221966LP

DNMT3B - human gene knockout kit via CRISPR, HDR mediated

Product images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: DNMT3B
• Locus ID: 1789
• Kit Components:

  KN221966G1, DNMT3B gRNA vector 1 in pCas-Guide CRISPR vector

  KN221966G2, DNMT3B gRNA vector 2 in pCas-Guide CRISPR vector

  KN221966LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001207055, NM_001207056, NM_006892, NM_175848, NM_175849, NM_175850
• UniProt ID: Q9UBC3
• Synonyms: ICF; ICF1; M.HsaIIIB
• Summary: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]