IRE1 (ERN1) Human Gene Knockout Kit (CRISPR)

CAT#: KN215023LP

ERN1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: IRE1
• Locus ID: 2081
• Components:

  KN215023G1, IRE1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN215023G2, IRE1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN215023LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGTTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC CTTCCCCTTC CTCTTCCAGC CCTTCCTCTT TCACTGACTG ACTGACTGGA AGACACACCT TTCGCTCCGG AGGTCGCCTC GGTAAACCTT GCGGATGATT CCACCACGGC GGAAAGACGA AAAGGAGGCT TCTAGGCACC CGGGACCGCC CGTGTCCCGC CCTTTTCCCC GATGAAGTCT GACGCGGCAG GTCCCTCGGC AGGGTTAATG GGACGGCCTG GTGGGGCATT GCCGGTCTTT CCCAGCCACA GACAAGCTTC GCGCCTCGAT CCCCCAGCGC CCCCAGACGC TGGATGCCGA GGCCTGGTCC TTTACAGCCG CCGACGCCTC ATGAGCGAAG CCAGCCAGAT GCCAGCACCG CCTTCGGCCC CGCCCCGAGC CAGCCCCTCC CACATCCCCG CCCGGGGCAC GCCCTCGGCC CGCCCCCATC TCCGCCCCGA ACACGCCCCT AGATTGGCCC CGCCCCGTCC CCGCCCGCAA CTCCGCCCCG ACCGGAAGAA GCCGCGGCAC CCAGCGCTTA TAGGGCCGGG AATGGGGGTG CCGCCGCTGC CTAGTCAGTT CTGCGTCCGC TGAGGCTCGG TCACCGCCTC GCTGTCGTCG CGGCGCCCCC GCCCCGTCCT CTGTCCGTAC CGCCCCCGGA GCCAGGGCCG AGTCCTCGCC ACTAGCATGG AAGACGCCAA AAACATAAAG AAAGGCCCAG CGCCATTCTA CCCACTCGAA GACGGGACCG CCGGCGAGCA GCTGCACAAA GCCATGAAGC GCTACGCCCT GGTGCCCGGC ACCATCGCCT TTACCGACGC ACATATCGAG GTGGACATTA CCTACGCCGA GTACTTCGAG ATGAGCGTTC GGCTGGCAGA AGCTATGAAG CGCTATGGGC TGAATACAAA CCATCGGATC GTGGTGTGCA GCGAGAATAG CTTGCAGTTC TTCATGCCCG TGTTGGGTGC CCTGTTCATC GGTGTGGCTG TGGCCCCAGC TAACGACATC TACAACGAGC GCGAGCTGCT GAACAGCATG GGCATCAGCC AGCCCACCGT CGTATTCGTG AGCAAGAAAG GGCTGCAAAA GATCCTCAAC GTGCAAAAGA AGCTACCGAT CATACAAAAG ATCATCATCA TGGATAGCAA GACCGACTAC CAGGGCTTCC AAAGCATGTA CACCTTCGTG ACTTCCCATT TGCCACCCGG CTTCAACGAG TACGACTTCG TGCCCGAGAG CTTCGACCGG GACAAAACCA TCGCCCTGAT CATGAACAGT AGTGGCAGTA CCGGATTGCC CAAGGGCGTA GCCCTACCGC ACCGCACCGC TTGTGTCCGA TTCAGTCATG CCCGCGACCC CATCTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTC AGCGTGGTGC CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTTCTT CGCTAAGAGC ACTCTCATCG ACAAGTACGA CCTAAGCAAC TTGCACGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG AGGTAGGTGA GGCCGTGGCC AAACGCTTCC ACCTACCAGG CATCCGCCAG GGCTACGGCC TGACAGAAAC AACCAGCGCC ATTCTGATCA CCCCCGAAGG GGACGACAAG CCTGGCGCAG TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTGGA CTTGGACACC GGTAAGACAC TGGGTGTGAA CCAGCGCGGC GAGCTGTGCG TCCGTGGCCC CATGATCATG AGCGGCTACG TTAACAACCC CGAGGCTACA AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GAGCATCCTG CTGCAACACC CCAACATCTT CGACGCCGGG GTCGCCGGCC TGCCCGACGA CGATGCCGGC GAGCTGCCCG CCGCAGTCGT CGTGCTGGAA CACGGTAAAA CCATGACCGA GAAGGAGATC GTGGACTATG TGGCCAGCCA GGTTACAACC GCCAAGAAGC TGCGCGGTGG TGTTGTGTTC GTGGACGAGG TGCCTAAAGG ACTGACCGGC AAGTTGGACG CCCGCAAGAT CCGCGAGATT CTCATTAAGG CCAAGAAGGG CGGAAAGATC GCCGTGTAAG AATTCCGATC ATATTCAATA ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTTAG GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGACCTGCA TCCATCTAGA TCTCGAGCAG CTGAAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCAC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATATCAGA TCCCCGGGAT GCAGAAATTG ATGATCTATT AAACAATAAA GATGTCCACT AAAATGGAAG TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG CAAAACCAAA TTAAGGGCCA GCTCATTCCT CCCACTCATG ATCTATAGAT CTATAGATCT CTCGTGGGAT CATTGTTTTT CTCTTGATTC CCACTTTGTG GTTCTAAGTA CTGTGGTTTC CAAATGTGTC AGTTTCATAG CCTGAAGAAC GAGATCAGCA GCCTCTGTTC CACATACACT TCATTCTCAG TATTGTTTTG CCAAGTTCTA ATTCCATCAG AAGCTGGTCG AGATCCGGAA CCCTTAATAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATTAGGTCCC TCGAAGAGGT TCACTAGGCG CGCCCGGGGC AGCGTCGCGG CGCCGGGGCT GGAGTCCGAG CTGGGAGCGC GACGGCGGCG GCCAGATGCC CCGTGAGCCC AGCCGTGGGA GGCTCGGGCC GGGCGTAGGA GGCGGCACCG GGCCGGGAGA GCTTCCCTGA CGGGAGTCGC GGCCCCACCG AGGCGTCGGC CGCCCGAAGA GCCTCCCAAG CCAGCGCGGC TGTCACGGGC AGCGGCTCCC CGGACCCCGC GGGCTCGGGC GCAACTTCGT GGCGTCCTGC ACGGCACTTG GGGGTCCCAG AAGGCCCCGG CCTGAGGAGG CCGCCCACCT GCGGGACCCT CCTCCTCGCG GGCGGGCGCC GGCGCTGCCT GGCGGGGGCC GCGGAGGAGC CGAAAGGGCG TTGGGATCCA GGGGAAGGGT CTGTCCTGCG CTTCGAATCC TTGTTTGAAC TTTTTAAAAA TTCGAAAACT TTGGGGAACG AATGCAACAG GGAGTAAGGA CTCTCACCCG AGAGCTGGCT TCTGCCACTT GCCCTGCCGG TTTCTTGGTA CTGGGAGGCT GCAGGCTTCC TTCTGTGCAC CGAGACTTCC TTGAGCAAGT GGAGCTGTGG GAATTGCACT CTTGACGACA GTCTTCACTG ACTGACTGAC TGGAAAGTCC TCTCCACTGA CTGTAGCCTC CAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCAACGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGACCC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001433, NM_152461
• UniProt ID: O75460
• Synonyms: hIRE1p; IRE1; IRE1a; IRE1P
• Summary: This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]