IRE1 (ERN1) (NM_001433) Human Tagged ORF Clone

CAT#: RC215023

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  • TrueORF®

ERN1 (Myc-DDK-tagged)-Human endoplasmic reticulum to nucleus signaling 1 (ERN1)



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ERN1
Synonyms hIRE1p; IRE1; IRE1a; IRE1P
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC215023 representing NM_001433
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCCCGGCGGCTGCTGCTGCTGCTGACGCTGCTGCTGCCCGGCCTCGGGATTTTTGGAAGTACCA
GCACAGTGACGCTTCCTGAAACCTTGTTGTTTGTGTCAACGCTGGATGGAAGTTTGCATGCTGTCAGCAA
GAGGACAGGCTCAATCAAATGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACATGTGGAAGAG
CCTGCCTTTCTCCCAGATCCTAATGATGGCAGCCTGTATACGCTTGGAAGCAAGAATAATGAAGGCCTGA
CGAAACTTCCTTTTACCATCCCAGAATTGGTGCAGGCATCCCCATGCCGAAGTTCAGATGGAATCCTCTA
CATGGGTAAAAAGCAGGACATCTGGTATGTTATTGACCTCCTGACCGGAGAGAAGCAGCAGACTTTGTCA
TCGGCCTTTGCAGATAGTCTCTGCCCATCAACCTCTCTTCTGTATCTTGGGCGAACAGAATACACCATCA
CCATGTACGACACCAAAACCCGAGAGCTCCGGTGGAATGCCACCTACTTTGACTATGCGGCCTCACTGCC
TGAGGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGTGATGGGCTGGTGGTGACTGTGGAC
AGTGAATCTGGGGACGTCCTATGGATCCAAAACTACGCCTCCCCTGTGGTGGCCTTTTATGTCTGGCAGC
GGGAGGGTCTGAGGAAGGTGATGCACATCAATGTCGCTGTGGAGACCCTGCGCTATCTGACCTTCATGTC
TGGGGAGGTGGGGCGCATCACAAAGTGGAAGTACCCGTTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTG
ACGCCCACTCTGTATGTTGGGAAATACTCTACCAGCCTCTATGCCTCTCCCTCAATGGTACACGAGGGGG
TTGCTGTCGTGCCCCGCGGCAGCACACTTCCTTTGCTGGAAGGGCCCCAGACTGATGGCGTCACCATCGG
GGACAAGGGGGAGTGTGTGATCACGCCCAGCACGGACGTCAAGTTTGATCCCGGACTCAAAAGCAAGAAC
AAGCTCAACTACTTGAGGAATTACTGGCTTCTGATAGGACACCATGAAACCCCACTGTCTGCGTCTACCA
AGATGCTGGAGAGATTTCCCAACAATCTACCCAAACATCGGGAAAATGTGATTCCTGCTGATTCAGAGAA
AAAGAGCTTTGAGGAAGTTATCAACCTGGTTGACCAGACTTCAGAAAACGCACCTACCACCGTGTCTCGG
GATGTGGAGGAGAAGCCCGCCCATGCCCCTGCCCGGCCCGAGGCCCCCGTGGACTCCATGCTTAAGGACA
TGGCTACCATCATCCTGAGCACCTTCCTGCTGATTGGCTGGGTGGCCTTCATCATCACCTATCCCCTGAG
CATGCATCAGCAGCAGCAGCTCCAGCACCAGCAGTTCCAGAAGGAACTGGAGAAGATCCAGCTCCTGCAG
CAGCAGCAGCAGCAGCTGCCCTTCCACCCACCTGGAGACACGGCTCAGGACGGCGAGCTCCTGGACACGT
CTGGCCCGTACTCAGAGAGCTCGGGCACCAGCAGCCCCAGCACGTCCCCCAGGGCCTCCAACCACTCGCT
CTGCTCCGGCAGCTCTGCCTCCAAGGCTGGCAGCAGCCCCTCCCTGGAACAAGACGATGGAGATGAGGAA
ACCAGCGTGGTGATAGTTGGGAAAATTTCCTTCTGTCCCAAGGATGTCCTGGGCCATGGAGCTGAGGGCA
CAATTGTGTACCGGGGCATGTTTGACAACCGCGACGTGGCCGTGAAGAGGATCCTCCCCGAGTGTTTTAG
CTTCGCAGACCGTGAGGTCCAGCTGTTGCGAGAATCGGATGAGCACCCGAACGTGATCCGCTACTTCTGC
ACGGAGAAGGACCGGCAATTCCAGTACATTGCCATCGAGCTGTGTGCAGCCACCCTGCAAGAGTATGTGG
AGCAGAAGGACTTTGCGCATCTCGGCCTGGAGCCCATCACCTTGCTGCAGCAGACCACCTCGGGCCTGGC
CCACCTCCACTCCCTCAACATCGTTCACAGAGACCTAAAGCCACACAACATCCTCATATCCATGCCCAAT
GCACACGGCAAGATCAAGGCCATGATCTCCGACTTTGGCCTCTGCAAGAAGCTGGCAGTGGGCAGACACA
GTTTCAGCCGCCGATCTGGGGTGCCTGGCACAGAAGGCTGGATCGCTCCAGAGATGCTGAGCGAAGACTG
TAAGGAGAACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGCGTCTTTTACTACGTAATCTCTGAG
GGCAGCCACCCTTTTGGCAAGTCCCTGCAGCGGCAGGCCAACATCCTCCTGGGTGCCTGCAGCCTTGACT
GCTTGCACCCAGAGAAGCACGAAGACGTCATTGCACGTGAATTGATAGAGAAGATGATTGCGATGGATCC
TCAGAAACGCCCCTCAGCGAAGCACGTGCTCAAACACCCGTTCTTCTGGAGCCTAGAGAAGCAGCTCCAG
TTCTTCCAGGACGTGAGCGACAGAATAGAAAAGGAATCCCTGGATGGCCCGATCGTGAAGCAGTTAGAGA
GAGGCGGGAGAGCCGTGGTGAAGATGGACTGGCGGGAGAACATCACTGTCCCCCTCCAGACAGACCTGCG
TAAATTCAGGACCTATAAAGGTGGTTCTGTCAGAGATCTCCTCCGAGCCATGAGAAATAAGAAGCACCAC
TACCGGGAGCTGCCTGCAGAGGTGCGGGAGACGCTGGGGTCCCTCCCCGACGACTTCGTGTGCTACTTCA
CATCTCGCTTCCCCCACCTCCTCGCACACACCTACCGGGCCATGGAGCTGTGCAGCCACGAGAGACTCTT
CCAGCCCTACTACTTCCACGAGCCCCCAGAGCCCCAGCCCCCAGTGACTCCAGACGCCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215023 representing NM_001433
Red=Cloning site Green=Tags(s)

MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEE
PAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLS
SAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVD
SESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKL
TPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKN
KLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSR
DVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQ
QQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEE
TSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFC
TEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPN
AHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISE
GSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQ
FFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHH
YRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001433
ORF Size 2931 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001433.5
RefSeq Size 3620 bp
RefSeq ORF 2934 bp
Locus ID 2081
UniProt ID O75460
Cytogenetics 17q23.3
Domains pkinase, TyrKc, S_TKc, PQQ, PUG
Protein Families Protein Kinase, Transmembrane
Protein Pathways Alzheimer's disease
MW 109.6 kDa
Gene Summary This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
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