ORF15 (NC_001348) Virus Tagged ORF Clone

CAT#: VC100956

Myc-DDK-tagged ORF clone of viral ORF for envelope protein UL43 [Human herpesvirus 3], codon optimized for human cell expression, NP_040138

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100956 represents NCBI reference of NP_040138 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTAAACGGCGAGAGGGCCGTACACGATGAAAACCTGGGTGTACTGGACCGGGAGTTGATACGGG
  CGCAGAGCATACAAGGATGTGTCGGCAACCCACAGGAATGTAATTCTTGTGCCATCACCAGCGCTTCCAG
  GCTTTTCCTGGTGGGACTCCAGGCGTCAGTGATCACCTCCGGACTGATCCTCCAGTACCATGTGTGTGAA
  GCTGCAGTCAACGCAACTATAATGGGTTTGATTGTTGTCAGCGGGCTGTGGCCGACGAGCGTGAAATTCC
  TGAGGACGCTGGCTAAGTTGGGTAGGTGCCTCCAGACAGTGGTCGTTCTGGGGTTCGCAGTGTTGTGGGC
  CGTTGGGTGTCCCATCAGCAGAGACCTGCCCTTTGTGGAACTGCTGGGCATCTCTATTTCTGCCATTACA
  GGGACAGTCGCAGCCGTGCATATACACTATTATAATTTTGTGACTACCTTTAACGGCCCCCATATTTATT
  TCTACGTCATGATGCTTGGAACAGGCCTTGGAGGGCTCCTGACAGTGATATTGTATATGTACGTAAGCAA
  ATATGAGGTGTTGATCGGCCTCTGCATTTCAATTGTGACTCTGGTAAGCATCGTGGACGCCGCGACTGAC
  CTCCAAGATACCTGCATTTATAGGAAGAACCGACATAAGCAGTTGAACACTTATACTGACTTGGGGTTTG
  CTGTCGTGTACACCCAGAATGATCGGGGCAGGGTGTGTGATCACAGGGAATCATCCCGCACGCTGAAAAG
  AGTCTTTAAGGGAATAAGAATTATGTCCGTCATTCCCCCCGTGCTGTATATTGTGACCCCTCTGATGTGG
  GCCATATCCCATATCATTAAACTTAACCACTTTATTAAGCTGACCCAAGTTACACTCGCAGTCTCAATTG
  GCGGTCACATTATCGCCTTTGGGCTGCAGGGATTTGCCGTCCTGTACCAGGAGAAGAAAAACCTGTGGGT
  GATCGTCCTCTACACCACAACTAGCGTGACCGGAATCGCTGTGACGTTCGCCGGGATATCTTGGGGCGCC
  ATTATTATCCTGACATCCACCGTGGCCGCCGGATTGACATGCATTCAGATGATGAGACTCAGCGTTAAGC
  CAATCGACTGCTTCATGGCAAGCCACATAACCAAAGTATACCATGTTTGTGTATACATAATCATAAATCT
  CTGCTATCTGTGTGGGACGTATGTGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100956 representing NP_040138
  Red=Cloning sites Green=Tags
  
  MAVNGERAVHDENLGVLDRELIRAQSIQGCVGNPQECNSCAITSASRLFLVGLQASVITSGLILQYHVCE
  AAVNATIMGLIVVSGLWPTSVKFLRTLAKLGRCLQTVVVLGFAVLWAVGCPISRDLPFVELLGISISAIT
  GTVAAVHIHYYNFVTTFNGPHIYFYVMMLGTGLGGLLTVILYMYVSKYEVLIGLCISIVTLVSIVDAATD
  LQDTCIYRKNRHKQLNTYTDLGFAVVYTQNDRGRVCDHRESSRTLKRVFKGIRIMSVIPPVLYIVTPLMW
  AISHIIKLNHFIKLTQVTLAVSIGGHIIAFGLQGFAVLYQEKKNLWVIVLYTTTSVTGIAVTFAGISWGA
  IIILTSTVAAGLTCIQMMRLSVKPIDCFMASHITKVYHVCVYIIINLCYLCGTYVS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001348.1, NP_040138
• RefSeq ORF: 1218 bp
• Locus ID: 1487652
• UniProt ID: P09310
• MW: 44.5 kDa