ORF33 (NC_001348) Virus Tagged ORF Clone

CAT#: VC100974

Myc-DDK-tagged ORF clone of viral ORF for capsid maturation protease [Human herpesvirus 3], codon optimized for human cell expression, NP_040156

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100974 represents NCBI reference of NP_040156 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGAGGCCGACGAGGAGAATTGCGAGGCTCTGTATGTGGCTGGATATCTCGCACTGTATTCCA
  AGGATGAGGGAGAGCTGAATATTACCCCTGAGATTGTAAGGTCAGCCCTGCCTCCCACAAGTAAAATTCC
  CATAAATATAGATCATAGGAAGGACTGCGTAGTGGGAGAGGTAATCGCAATTATCGAGGACATACGCGGC
  CCCTTCTTCCTGGGTATCGTGCGATGTCCACAGCTTCATGCTGTTCTTTTTGAGGCAGCCCATAGCAACT
  TCTTCGGAAACAGGGATAGTGTGCTGAGTCCCCTCGAGAGAGCTCTCTATCTCGTTACCAACTATCTGCC
  TTCCGTGAGTCTGAGCAGTAAACGGCTGAGTCCTAATGAAATTCCGGACGGGAACTTCTTCACGCATGTG
  GCTTTGTGCGTGGTTGGCCGGCGAGTCGGGACAGTTGTTAATTATGACTGCACCCCAGAATCCAGCATCG
  AACCCTTTCGCGTCCTGTCAATGGAAAGTAAGGCCAGGCTGCTCTCACTGGTGAAAGACTACGCCGGACT
  TAATAAAGTTTGGAAGGTTAGCGAAGATAAGCTCGCAAAGGTACTGCTGTCCACTGCTGTAAATAACATG
  TTGCTGCGCGACAGATGGGACGTCGTTGCCAAAAGGCGCAGGGAGGCTGGGATCATGGGCCACGTTTACC
  TGCAGGCCAGTACTGGGTATGGTTTGGCTCGCATCACAAACGTCAACGGCGTGGAGAGTAAGCTTCCAAA
  CGCCGGTGTAATTAATGCCACCTTCCATCCTGGAGGCCCGATATATGATTTGGCTCTGGGCGTTGGGGAG
  AGCAACGAGGACTGCGAAAAGACTGTACCACACCTGAAGGTTACACAATTGTGCAGAAATGACTCCGACA
  TGGCCTCAGTCGCCGGAAACGCCAGCAACATAAGTCCCCAGCCACCCAGTGGCGTCCCCACGGGCGGGGA
  GTTTGTCTTGATTCCCACTGCTTACTACTCACAACTGCTCACCGGACAGACTAAAAATCCGCAGGTCAGC
  ATTGGCGCCCCAAACAATGGGCAGTATATAGTCGGCCCATATGGCTCACCTCATCCACCGGCCTTCCCCC
  CTAACACTGGCGGATACGGTTGCCCCCCCGGGCACTTCGGAGGCCCCTATGGGTTTCCAGGATACCCCCC
  GCCCAATCGCCTTGAGATGCAAATGTCTGCTTTCATGAACGCGCTCGCAGCGGAGAGAGGCATTGACCTC
  CAGACCCCATGCGTCAATTTCCCCGACAAGACCGATGTCAGGAGGCCAGGCAAGAGGGACTTCAAATCTA
  TGGACCAGCGGGAACTGGACTCCTTCTATAGCGGAGAGTCTCAAATGGACGGCGAATTCCCCTCTAATAT
  CTACTTCCCGGGAGAGCCTACATATATCACGCACCGACGGCGGAGGGTGTCTCCAAGTTACTGGCAAAGG
  AGGCACAGAGTCTCTAACGGACAGCACGAGGAACTGGCCGGGGTCGTGGCCAAACTCCAACAAGAGGTGA
  CCGAACTGAAGAGCCAGAACGGCACCCAGATGCCCCTGTCCCACCACACCAATATCCCAGAGGGCACAAG
  GGACCCCAGAATCTCTATACTCCTGAAGCAGCTGCAGTCCGTGAGCGGCTTGTGTTCTTCTCAGAACACA
  ACATCCACCCCACACACCGACACAGTTGGGCAGGACGTCAATGCCGTGGAAGCTAGCTCAAAAGCTCCAC
  TCATCCAGGGCAGCACCGCTGATGACGCTGACATGTTCGCGAACCAGATGATGGTCGGGCGGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100974 representing NP_040156
  Red=Cloning sites Green=Tags
  
  MAAEADEENCEALYVAGYLALYSKDEGELNITPEIVRSALPPTSKIPINIDHRKDCVVGEVIAIIEDIRG
  PFFLGIVRCPQLHAVLFEAAHSNFFGNRDSVLSPLERALYLVTNYLPSVSLSSKRLSPNEIPDGNFFTHV
  ALCVVGRRVGTVVNYDCTPESSIEPFRVLSMESKARLLSLVKDYAGLNKVWKVSEDKLAKVLLSTAVNNM
  LLRDRWDVVAKRRREAGIMGHVYLQASTGYGLARITNVNGVESKLPNAGVINATFHPGGPIYDLALGVGE
  SNEDCEKTVPHLKVTQLCRNDSDMASVAGNASNISPQPPSGVPTGGEFVLIPTAYYSQLLTGQTKNPQVS
  IGAPNNGQYIVGPYGSPHPPAFPPNTGGYGCPPGHFGGPYGFPGYPPPNRLEMQMSAFMNALAAERGIDL
  QTPCVNFPDKTDVRRPGKRDFKSMDQRELDSFYSGESQMDGEFPSNIYFPGEPTYITHRRRRVSPSYWQR
  RHRVSNGQHEELAGVVAKLQQEVTELKSQNGTQMPLSHHTNIPEGTRDPRISILLKQLQSVSGLCSSQNT
  TSTPHTDTVGQDVNAVEASSKAPLIQGSTADDADMFANQMMVGRC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001348.1, NP_040156
• RefSeq ORF: 1815 bp
• Locus ID: 1487717
• UniProt ID: P09310
• MW: 66.0 kDa