L1 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100376

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 52K [Human adenovirus C], codon optimized for human cell expression, NP_040519

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100376 represents NCBI reference of NP_040519 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCTGTGTTGCGCCAAATGCGGCCCCCCCCACAGCAGAGGCAGGAGCAAGAACAACGCCAGACGT
  GTCGCGCGCCATCTCCATCACCTACGGCCTCCGGCGGCGCGACCAGTGCTGCAGATGCTGCTGCCGATGG
  TGATTATGAGCCGCCCAGGAGACGAGCCCGCCACTATCTTGACCTTGAGGAGGGGGAAGGTCTGGCTAGG
  CTGGGCGCCCCATCCCCCGAGAGACACCCTCGGGTTCAGCTGAAAAGAGACACAAGGGAAGCTTACGTTC
  CTCGCCAGAACTTGTTCCGGGATCGAGAAGGCGAAGAACCTGAGGAGATGAGAGACAGAAAGTTTCATGC
  AGGACGCGAGCTGAGACACGGTCTTAATCGGGAACGGCTCCTGCGAGAAGAGGATTTCGAACCTGACGCA
  AGAACAGGAATAAGCCCTGCTAGGGCTCACGTGGCGGCAGCGGACCTCGTTACCGCCTATGAGCAGACCG
  TGAACCAGGAAATTAATTTCCAGAAAAGTTTTAATAACCACGTGAGAACACTCGTAGCCAGAGAAGAGGT
  GGCAATAGGCCTGATGCACCTGTGGGACTTTGTTAGTGCCTTGGAGCAGAATCCCAATAGCAAGCCACTT
  ATGGCTCAGCTGTTCTTGATAGTCCAGCATAGTCGAGATAACGAGGCTTTCCGCGACGCTCTCCTTAATA
  TCGTGGAACCTGAAGGTAGATGGCTGCTGGACCTGATTAATATTCTCCAGTCCATCGTCGTGCAGGAAAG
  ATCCCTCTCACTGGCAGATAAGGTGGCCGCAATCAACTACTCCATGCTGAGCCTGGGTAAGTTTTACGCA
  CGGAAAATCTATCACACACCTTATGTACCAATTGACAAAGAAGTTAAGATCGAGGGGTTTTACATGAGAA
  TGGCCCTGAAGGTTCTGACACTGTCAGATGACCTCGGCGTGTATCGCAATGAACGCATCCATAAGGCTGT
  TTCCGTTTCTCGCCGCAGGGAGTTGTCCGATCGCGAGCTGATGCACTCATTGCAGAGAGCGCTGGCTGGC
  ACCGGTTCAGGAGATAGGGAGGCGGAATCCTACTTTGATGCAGGAGCAGACCTTAGGTGGGCACCTTCAA
  GAAGAGCTCTCGAGGCAGCAGGAGCTGGACCCGGCCTCGCAGTCGCACCCGCGCGGGCTGGGAATGTTGG
  CGGCGTTGAGGAGTACGATGAAGACGACGAATATGAGCCCGAGGATGGAGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100376 representing NP_040519
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPPPQQRQEQEQRQTCRAPSPSPTASGGATSAADAAADGDYEPPRRRARHYLDLEEGEGLAR
  LGAPSPERHPRVQLKRDTREAYVPRQNLFRDREGEEPEEMRDRKFHAGRELRHGLNRERLLREEDFEPDA
  RTGISPARAHVAAADLVTAYEQTVNQEINFQKSFNNHVRTLVAREEVAIGLMHLWDFVSALEQNPNSKPL
  MAQLFLIVQHSRDNEAFRDALLNIVEPEGRWLLDLINILQSIVVQERSLSLADKVAAINYSMLSLGKFYA
  RKIYHTPYVPIDKEVKIEGFYMRMALKVLTLSDDLGVYRNERIHKAVSVSRRRELSDRELMHSLQRALAG
  TGSGDREAESYFDAGADLRWAPSRRALEAAGAGPGLAVAPARAGNVGGVEEYDEDDEYEPEDGEY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001405.1, NP_040519
• RefSeq ORF: 1245 bp
• Locus ID: 2652993
• MW: 47.0 kDa