L2 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100378

Myc-DDK-tagged ORF clone of viral ORF for penton base [Human adenovirus C], codon optimized for human cell expression, NP_040521

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100378 represents NCBI reference of NP_040521 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGGCAGCGATGTACGAAGAAGGGCCACCTCCCTCCTATGAGAGTGTGGTTTCAGCCGCGCCAG
  TGGCTGCAGCCCTTGGCAGCCCATTTGATGCTCCCCTTGACCCACCATTCGTTCCACCCCGGTACCTGCG
  GCCGACAGGCGGACGGAACTCCATCAGGTATTCCGAACTGGCGCCACTGTTCGACACCACTCGAGTGTAT
  CTCGTTGATAATAAAAGCACAGATGTCGCATCTCTTAACTATCAGAACGACCACAGCAACTTCCTGACTA
  CAGTAATTCAGAACAACGATTATAGTCCCGGAGAAGCCAGTACTCAAACAATCAATCTTGATGACCGGAG
  CCATTGGGGCGGCGATCTTAAAACCATCCTGCACACCAATATGCCCAATGTGAACGAGTTTATGTTTACC
  AACAAGTTCAAAGCTAGGGTGATGGTTAGTCGAAGCCTGACTAAGGATAAGCAAGTGGAGCTGAAATATG
  AGTGGGTTGAATTTACGCTGCCTGAGGGAAACTACAGTGAGACAATGACTATCGACCTGATGAATAACGC
  TATTGTGGAACATTATCTGAAGGTGGGGCGCCAGAACGGGGTCCTGGAGTCTGACATTGGCGTTAAATTC
  GACACCCGAAACTTTCGCCTTGGCTTCGACCCTGTCACTGGGCTGGTGATGCCCGGCGTCTATACGAACG
  AGGCTTTCCACCCCGACATCATCCTGCTTCCAGGCTGCGGGGTAGATTTTACGCATAGCCGACTGAGCAA
  CCTCCTTGGCATCAGAAAACGACAGCCCTTCCAGGAAGGATTCAGAATCACCTATGACGACCTTGAAGGG
  GGCAACATCCCCGCGCTTCTCGATGTTGATGCCTACCAGGCATCCCTGAAGGACGATACCGAGCAAGGCG
  GCGATGGAGCTGGAGGAGGCAATAACAGTGGGTCAGGGGCTGAAGAGAACAGCAACGCAGCTGCGGCAGC
  CATGCAGCCAGTCGAAGATATGAATGATCATGCAATCCGGGGAGATACTTTTGCAACACGCGCCGAGGAG
  AAACGCGCCGAGGCTGAGGCTGCAGCCGAGGCCGCCGCCCCCGCCGCTCAGCCAGAAGTGGAAAAACCCC
  AGAAGAAACCCGTGATCAAGCCACTGACAGAAGACTCTAAAAAGCGAAGTTATAATCTGATATCAAATGA
  CAGCACCTTCACCCAGTACCGCTCCTGGTATCTGGCCTACAACTACGGGGATCCTCAGACCGGAATTCGG
  TCTTGGACACTTCTGTGTACTCCAGATGTCACATGTGGCAGCGAGCAAGTTTATTGGAGCCTGCCCGACA
  TGATGCAGGATCCAGTGACGTTCCGGAGCACGAGTCAGATCTCAAATTTCCCCGTTGTTGGCGCCGAGCT
  GCTTCCCGTACACTCCAAATCATTTTACAACGATCAAGCGGTATACTCTCAGCTCATTCGACAGTTTACA
  AGCCTGACGCATGTTTTCAATCGATTCCCAGAAAACCAGATCTTGGCAAGACCTCCAGCGCCCACAATCA
  CAACCGTGAGCGAAAATGTCCCAGCCCTCACTGACCACGGGACACTCCCCTTGAGGAATTCCATCGGCGG
  CGTGCAGCGCGTAACCATCACCGACGCTAGGCGGAGGACCTGTCCCTACGTGTACAAAGCGCTCGGGATT
  GTGTCCCCCAGAGTGCTCAGTTCACGGACATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100378 representing NP_040521
  Red=Cloning sites Green=Tags
  
  MQRAAMYEEGPPPSYESVVSAAPVAAALGSPFDAPLDPPFVPPRYLRPTGGRNSIRYSELAPLFDTTRVY
  LVDNKSTDVASLNYQNDHSNFLTTVIQNNDYSPGEASTQTINLDDRSHWGGDLKTILHTNMPNVNEFMFT
  NKFKARVMVSRSLTKDKQVELKYEWVEFTLPEGNYSETMTIDLMNNAIVEHYLKVGRQNGVLESDIGVKF
  DTRNFRLGFDPVTGLVMPGVYTNEAFHPDIILLPGCGVDFTHSRLSNLLGIRKRQPFQEGFRITYDDLEG
  GNIPALLDVDAYQASLKDDTEQGGDGAGGGNNSGSGAEENSNAAAAAMQPVEDMNDHAIRGDTFATRAEE
  KRAEAEAAAEAAAPAAQPEVEKPQKKPVIKPLTEDSKKRSYNLISNDSTFTQYRSWYLAYNYGDPQTGIR
  SWTLLCTPDVTCGSEQVYWSLPDMMQDPVTFRSTSQISNFPVVGAELLPVHSKSFYNDQAVYSQLIRQFT
  SLTHVFNRFPENQILARPPAPTITTVSENVPALTDHGTLPLRNSIGGVQRVTITDARRRTCPYVYKALGI
  VSPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001405
• ORF Size: 1713 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001405.1, NP_040521
• RefSeq ORF: 1713 bp
• Locus ID: 2652996
• MW: 63.3 kDa