L2 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100264

Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040920

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100264 represents NCBI reference of NP_040920 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGAGCAGTCGAACTTCAGACAGTGGCCTTCCCCGAAACACCCCCTCCTAGTTACGAAACAGTAA
  TGGCTGCGGCCCCCCCTTATGTGCCGCCAAGATACCTCGGACCAACAGAGGGGAGGAATAGCATCCGATA
  TTCTGAACTGTCACCTTTGTACGATACCACACGGGTCTACCTGGTGGATAACAAATCCAGCGATATCGCT
  TCACTGAACTACCAGAACGATCACAGCAACTTTCTCACAACTGTGGTGCAGAATAATGATTACTCTCCGA
  TCGAAGCTGGGACACAGACCATCAACTTCGATGAACGGAGCAGATGGGGCGGGGATTTGAAAACAATTCT
  GCACACGAACATGCCTAATGTCAACGACTTCATGTTTACAACAAAGTTCAAGGCCAGGGTAATGGTCGCC
  AGAAAAACTAACAATGAAGGGCAAACTATCCTCGAGTACGAATGGGCCGAATTTGTCTTGCCCGAGGGGA
  ATTACAGTGAAACAATGACCATTGATCTTATGAATAATGCCATTATTGAACACTATCTCCGCGTGGGCAG
  ACAGCATGGCGTTTTGGAAAGCGATATCGGTGTAAAGTTTGATACAAGAAACTTCAGACTGGGGTGGGAC
  CCAGAGACCCAGCTCGTAACCCCTGGGGTATACACCAACGAAGCGTTTCATCCCGACATCGTATTGCTTC
  CCGGTTGTGGGGTTGATTTTACAGAGTCCAGACTGTCTAATATACTCGGCATTAGGAAAAGGCAGCCCTT
  TCAAGAGGGCTTCGTCATTATGTATGAACATCTCGAGGGCGGTAACATCCCCGCACTCCTCGACGTCAAG
  AAGTACGAGAACAGCCTTCAGGACCAGAACACAGTACGAGGGGATAACTTCATCGCTTTGAACAAGGCTG
  CAAGGATTGAACCAGTAGAGACAGACCCCAAGGGGAGGAGCTACAACCTTCTGCCAGACAAGAAGAATAC
  GAAGTACAGGTCATGGTATCTGGCGTACAATTATGGGGATCCGGAAAAGGGAGTGAGGTCCTGGACTTTG
  TTGACGACCCCTGATGTCACAGGGGGTAGTGAGCAGGTCTACTGGAGTCTGCCCGATATGATGCAGGACC
  CAGTGACATTTCGCTCCTCCCGGCAGGTAAGTAACTACCCTGTTGTCGCCGCGGAGCTGCTGCCAGTTCA
  CGCCAAATCCTTCTACAATGAGCAGGCTGTCTACTCACAGCTGATACGCCAGTCTACCGCCCTCACAAGA
  GTGTTCAACCGCTTCCCAGAGAATCAAATCCTGGTCAGACCTCCGGCCGCTACAATTACTACAGTAAGTG
  AGAACGTTCCAGCGCTGACAGATCACGGTACGCTTCCCCTTAGGTCTTCTATCTCTGGAGTTCAGAGGGT
  GACAATCACGGACGCCCGGAGACGCACCTGTCCATACGTCTACAAGGCCCTCGGTATTGTTTCTCCCAGG
  GTGCTGTCCAGTAGGACTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100264 representing NP_040920
  Red=Cloning sites Green=Tags
  
  MRRAVELQTVAFPETPPPSYETVMAAAPPYVPPRYLGPTEGRNSIRYSELSPLYDTTRVYLVDNKSSDIA
  SLNYQNDHSNFLTTVVQNNDYSPIEAGTQTINFDERSRWGGDLKTILHTNMPNVNDFMFTTKFKARVMVA
  RKTNNEGQTILEYEWAEFVLPEGNYSETMTIDLMNNAIIEHYLRVGRQHGVLESDIGVKFDTRNFRLGWD
  PETQLVTPGVYTNEAFHPDIVLLPGCGVDFTESRLSNILGIRKRQPFQEGFVIMYEHLEGGNIPALLDVK
  KYENSLQDQNTVRGDNFIALNKAARIEPVETDPKGRSYNLLPDKKNTKYRSWYLAYNYGDPEKGVRSWTL
  LTTPDVTGGSEQVYWSLPDMMQDPVTFRSSRQVSNYPVVAAELLPVHAKSFYNEQAVYSQLIRQSTALTR
  VFNRFPENQILVRPPAATITTVSENVPALTDHGTLPLRSSISGVQRVTITDARRRTCPYVYKALGIVSPR
  VLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001460.1, NP_040920
• RefSeq ORF: 1491 bp
• Locus ID: 1460868
• MW: 56.4 kDa