L1 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100263

Myc-DDK-tagged ORF clone of viral ORF for L1 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040919

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100263 represents NCBI reference of NP_040919 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACGGCCCGCAATCATCGCTGAGCGCGCACCTAATCTCGACCCAGCAGTGCTGGCCGCTATGCAGT
  CACAGCCTAGTGGAGTGACAGCCTCCGACGACTGGACCGCAGCCATGGATAGAATAATGGCACTGACAGC
  GCGCAGCCCGGACGCTTTTCGGCAGCAGCCGCAAGCTAACCGCTTTTCCGCCATTCTTGAAGCTGTTGTA
  CCGTCCCGCACCAACCCCACTCACGAAAAAGTGCTGACCATAGTGAACGCTCTCCTCGACTCCAAGGCCA
  TTCGAAAGGACGAGGCTGGCTTGATCTACAATGCTCTCCTGGAGCGCGTGGCCCGATACAACTCCACCAA
  CGTGCAGGCAAACCTTGATAGAATGGGAACCGACGTGAAGGAGGCACTGGCCCAGCGGGAACGGTTTCAT
  CGGGATGGAAATCTCGGGTCTCTGGTGGCTCTCAACGCATTTCTCAGCACTCAGCCGGCAAACGTTCCCC
  GCGGTCAGGAGGATTACACAAATTTCATTTCTGCTCTCAGGCTGATGGTAACAGAGGTGCCTCAGAGCGA
  GGTGTACCAGTCTGGGCCTGACTACTTTTTTCAGACATCCCGCCAGGGTCTGCAGACTGTGAATCTTACG
  CAAGCATTCAAGAACTTGCAGGGCCTGTGGGGGGTCCGCGCACCTGTGGGCGACAGGTCTACACTGAGCT
  CACTGCTCACCCCGAATAGCAGGCTTCTTCTGCTGCTGATCGCCCCATTTACAAATACGAACTCCCTGTC
  CAGGGACTCTTATCTCGGCCACTTGGTCACCCTGTATCGGGAAGCAATTGGCCAGGCGCAGGTCGATGAA
  CAGACCTACCAGGAAATTACTTCAGTAAGCAGAGCCCTGGGGCAGGAAGACACAGGATCCCTGGAAGCTA
  CCCTGAACTTCCTGCTGACGAATCGACGGCAGCAAGTTCCCCCGCAGTATACCCTGAATGCTGAGGAGGA
  GCGGATCCTCAGATATGTACAGCAGTCCGTTTCCTTGTATCTGATGAGGGAGGGCGCCACACCCAGCGCA
  GCTCTTGATATGACTGCTAGGAACATGGAGCCCAGCTTCTATGCTAGTAACCGAGCGTTCATCAACAGAC
  TGATGGACTATCTGCACAGAGCCGCCGCCATGAATGGGGAGTATTTTACTAACGCTATTCTGAATCCGCA
  TTGGCTGCCGCCTCCAGGTTTTTACACCGGCGAATTCGATTTGCCCGAGGGCAACGATGGTTTCCTCTGG
  GACGATGTTACAGATTCCCTCTTTTCACCAGCCGTGATCGGCCACCACGGCAAGAAAGAAGCCGGTGATG
  AGGGTCCCCTTCTTGACTCCAGAGCAAGCAGTCCTTTCCCATCTCTGACCAGCCTGCCCGCCAGTGTGAA
  TTCCGGCCGCACCACCCGACCTCGCCTTACAGGGGAGTCCGAGTATTTGAATGATCCGATTCTGTTCCCA
  GTGAGAGACAAGAACTTCCCCAATAATGGAATCGAATCCTTGGTCGATAAAATGAGTAGGTGGAAGACCT
  ATGCCCAAGAGCGCAGAGAATGGGAAGAACGGCAGCCCCGCCCAGTTCGACCCCCACGGCAAAGATGGCA
  GCGCCGGAAAAAGGGCGCCCACGCCGGGGACGAAGGGTCCGATGACAGTGCTGATGATAGCAGCGTATTG
  GATCTGGGGGGCAGTGGAAATCCATTCGCCCACCTCCGCCCACAGGGATGCATCGGGTCCCTCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100263 representing NP_040919
  Red=Cloning sites Green=Tags
  
  MQRPAIIAERAPNLDPAVLAAMQSQPSGVTASDDWTAAMDRIMALTARSPDAFRQQPQANRFSAILEAVV
  PSRTNPTHEKVLTIVNALLDSKAIRKDEAGLIYNALLERVARYNSTNVQANLDRMGTDVKEALAQRERFH
  RDGNLGSLVALNAFLSTQPANVPRGQEDYTNFISALRLMVTEVPQSEVYQSGPDYFFQTSRQGLQTVNLT
  QAFKNLQGLWGVRAPVGDRSTLSSLLTPNSRLLLLLIAPFTNTNSLSRDSYLGHLVTLYREAIGQAQVDE
  QTYQEITSVSRALGQEDTGSLEATLNFLLTNRRQQVPPQYTLNAEEERILRYVQQSVSLYLMREGATPSA
  ALDMTARNMEPSFYASNRAFINRLMDYLHRAAAMNGEYFTNAILNPHWLPPPGFYTGEFDLPEGNDGFLW
  DDVTDSLFSPAVIGHHGKKEAGDEGPLLDSRASSPFPSLTSLPASVNSGRTTRPRLTGESEYLNDPILFP
  VRDKNFPNNGIESLVDKMSRWKTYAQERREWEERQPRPVRPPRQRWQRRKKGAHAGDEGSDDSADDSSVL
  DLGGSGNPFAHLRPQGCIGSLY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001460
• ORF Size: 1746 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001460.1, NP_040919
• RefSeq ORF: 1746 bp
• Locus ID: 1460848
• MW: 64.8 kDa