PSMD2 (NM_001278708) Human Untagged Clone

CAT#: SC337437

PSMD2 (untagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD2
• Synonyms: P97; RPN1; S2; TRAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001278708, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCATGAGTGGGGAGCGTGAGTGCCTCAAGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCAT
  GGGGTCATGAGTATGTCAGGCATCTGGCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGA
  GAAGGTCCAGCGGGAGCCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCA
  GAGCATGAGGCTTGCGACCTGCTTATGGAAATTGAGCAGGTGGACATGCTGGAGAAGGACATTGATGAAA
  ATGCATATGCAAAGGTCTGCCTTTATCTCACCAGTTGTGTGAATTACGTGCCTGAGCCTGAGAACTCAGC
  CCTACTGCGTTGTGCCCTGGGTGTGTTCCGAAAGTTTAGCCGCTTCCCTGAAGCTCTGAGATTGGCATTG
  ATGCTCAATGACATGGAGTTGGTAGAAGACATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGA
  TGGCATTCATGCTAGGCCGGCATGGGGTGTTCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCT
  GACAGAGATCATGTCCAATGTACAGCTCAACAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATG
  GAGCCCAAGGTGCCTGATGACATCTACAAAACCCACCTAGAGAACAACAGGTTTGGGGGCAGTGGCTCTC
  AGGTGGACTCTGCCCGCATGAACCTGGCCTCCTCTTTTGTGAATGGCTTTGTGAATGCAGCTTTTGGCCA
  AGACAAGCTGCTAACAGATGATGGCAACAAATGGCTTTACAAGAACAAGGACCACGGAATGTTGAGTGCA
  GCTGCATCTCTTGGGATGATTCTGCTGTGGGATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGT
  ACTCCTCTGAGGACTACATTAAGTCAGGAGCTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAA
  TGAGTGTGACCCTGCTCTGGCACTGCTCTCAGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGT
  TCCATCTTTGGGCTAGGCTTGGCTTATGCTGGCTCAAATCGTGAAGATGTCCTAACACTGCTGCTGCCTG
  TGATGGGAGATTCAAAGTCCAGCATGGAGGTGGCAGGTGTCACAGCTTTAGCCTGTGGAATGATAGCAGT
  AGGGTCCTGCAATGGAGATGTAACTTCCACTATCCTTCAGACCATCATGGAGAAGTCAGAGACTGAGCTC
  AAGGATACTTATGCTCGTTGGCTTCCTCTTGGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATCG
  AGGCAATCCTGGCTGCACTGGAGGTTGTGTCAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGATGT
  GTGTGCATATGCAGGCTCTGGGAATGTGCTGAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACACTTT
  GACTCCAAAGAGAAGGAGGAAGACAAAGACAAGAAGGAAAAGAAAGACAAGGACAAGAAGGAAGCCCCTG
  CTGACATGGGAGCACATCAGGGAGTGGCTGTTCTGGGGATTGCCCTTATTGCTATGGGGGAGGAGATTGG
  TGCAGAGATGGCATTACGAACCTTTGGCCACTTGCTGAGATATGGGGAGCCTACACTCCGGAGGGCTGTA
  CCTTTAGCACTGGCCCTCATCTCTGTTTCAAATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCT
  CTCATGATGCTGATCCAGAAGTTTCCTATAACTCCATTTTTGCCATGGGCATGGTGGGCAGTGGTACCAA
  TAATGCCCGTCTGGCTGCAATGCTGCGCCAGTTAGCTCAATATCATGCCAAGGACCCAAACAACCTCTTC
  ATGGTGCGCTTGGCACAGGGCCTGACACATTTAGGGAAGGGCACCCTTACCCTCTGCCCCTACCACAGCG
  ACCGGCAGCTTATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTTGTCTCTTTCCTGGATGTTCG
  AAACATTATTCTAGGCAAATCACACTATGTATTGTATGGGCTGGTGGCTGCCATGCAGCCCCGAATGCTG
  GTTACGTTTGATGAGGAGCTGCGGCCATTGCCAGTGTCTGTCCGTGTGGGCCAGGCAGTGGATGTGGTGG
  GCCAGGCTGGCAAGCCGAAGACTATCACAGGGTTCCAGACGCATACAACCCCAGTGTTGTTGGCCCACGG
  GGAACGGGCAGAATTGGCCACTGAGGAGTTTCTTCCTGTTACCCCCATTCTGGAAGGTTTTGTTATCCTT
  CGGAAGAACCCCAATTATGATCTCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278708
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278708.1, NP_001265637.1
• RefSeq Size: 2655 bp
• RefSeq ORF: 2337 bp
• Locus ID: 5708
• UniProt ID: Q13200
• Cytogenetics: 3q27.1
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which is shorter at the N-terminus compared to isoform 1.