BRRN1 (NCAPH) (NM_001281710) Human Untagged Clone

CAT#: SC337324

NCAPH (untagged) - Human non-SMC condensin I complex, subunit H (NCAPH), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCAPH
• Synonyms: BRRN1; CAP-H; CAPH; MCPH23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001281710, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGACCTCCCGGCCCAGAGACGCGAGGACACCCCCACAGTGCCTCCTCTCCTTCAGAGCGTGTGTTCC
  CGATGCCCCTGCCCAGGAAGGCGCCTCTCAATATTCCTGGCACCCCAGTCCTCGAAGACTTTCCTCAGAA
  TGACGATGAGAAGGAGCGGCTGCAGCGGAGGCGCTCGAGGGTCTTTGATCTGCAGTTCAGCACTGACTCA
  CCTCGCTTATTGGCCTCCCCCTCCAGCAGGAGTATTGACATTTCAGCTACTATCCCCAAGTTTACAAACA
  CGCAGATTACGGAACATTACTCCACCTGTATCAAACTGTCCACTGAAAATAAAATCACTACCAAGAATGC
  TTTTGGTTTGCACTTGATTGATTTTATGTCAGAGATTCTTAAACAGAAAGACACCGAACCAACCAACTTT
  AAAGTGGCTGCGGGTACTCTGGATGCCAGCACCAAGATCTATGCTGTGCGCGTGGATGCCGTCCATGCCG
  ATGTATACAGAGTCCTTGGGGGGCTGGGCAAAGATGCACCGTCTTTGGAAGAAGTAGAAGGCCATGTTGC
  TGATGGAAGTGCTACTGAAATGGGAACAACCAAAAAGGCTGTAAAGCCAAAGAAGAAGCACTTACACAGA
  ACTATTGAGCAGAACATAAACAACCTCAATGTCTCCGAAGCAGATCGGAAGTGTGAGATTGATCCCATGT
  TTCAGAAGACAGCAGCCTCATTTGATGAGTGCAGCACAGCAGGGGTGTTTCTGTCCACTCTCCACTGCCA
  GGACTACAGAAGTGAACTGCTGTTTCCCTCTGATGTCCAGACTCTCTCCACGGGAGAACCTCTCGAGTTG
  CCAGAGTTAGGTTGTGTAGAAATGACAGATTTAAAAGCGCCCTTGCAGCAGTGTGCAGAAGATCGCCAGA
  TCTGCCCTTCCCTGGCCGGGTTCCAGTTTACACAGTGGGACAGTGAAACACATAATGAGTCTGTGTCGGC
  CCTGGTAGACAAGTTTAAGAAGAATGACCAGGTATTTGACATCAATGCTGAAGTTGACGAGAGTGACTGT
  GGAGACTTCCCCGATGGGTCCCTGGGGGATGACTTTGATGCCAACGATGAACCTGACCACACCGCAGTTG
  GGGATCATGAAGAGTTCAGGAGCTGGAAGGAGCCCTGCCAGGTTCAGAGCTGCCAGGAAGAAATGATTTC
  CCTTGGGGATGGAGACATCAGGACCATGTGCCCCCTTCTGTCTATGAAACCTGGAGAATATTCTTATTTC
  AGTCCTCGGACCATGTCGATGTGGGCTGGCCCGGATCACTGGCGCTTTAGGCCTCGACGCAAACAAGATG
  CTCCTTCCCAATCAGAAAACAAAAAGAAGAGTACAAAAAAAGATTTTGAAATTGACTTTGAAGATGATAT
  TGACTTTGATGTATATTTTAGAAAAACAAAGGCTGCTACTATTCTGACCAAGTCCACTTTGGAGAACCAG
  AATTGGAGAGCTACCACCCTTCCTACAGATTTCAACTACAATGTTGACACTCTGGTCCAGCTTCACCTCA
  AACCAGGCACCAGGTTACTTAAGATGGCCCAGGGCCATAGGGTAGAGACTGAGCATTATGAAGAAATTGA
  AGACTATGATTACAACAACCCTAACGACACCTCCAACTTTTGCCCTGGATTACAGGCTGCTGACAGTGAT
  GATGAAGATTTGGATGACTTATTTGTGGGACCTGTTGGGAACTCTGACCTCTCACCTTATCCTTGCCATC
  CACCTAAGACAGCACAACAGAATGGTGACACTCCAGAAGCCCAAGGATTAGACATCACAACATATGGGGA
  GTCAAACTTGGTAGCTGAGCCTCAGAAGGTAAATAAAATTGAAATTCACTATGCCAAGACTGCCAAAAAG
  ATGGACATGAAGAAACTGAAGCAGAGCATGTGGAGTCTGCTGACAGCGCTCTCCGGAAAGGAGGCAGATG
  CAGAGGCAAACCACAGGGAAGCTGGAAAAGAAGCGGCCCTGGCAGAAGTGGCTGACGAGAAGATGCTTAG
  CGGGCTCACGAAGGACCTGCAGAGGAGCCTGCCCCCTGTCATGGCTCAGAACCTCTCCATACCTCTGGCT
  TTTGCCTGTCTCCTACATTTAGCCAATGAAAAGAATCTAAAACTGGAAGGAACAGAGGACCTCTCTGATG
  TTCTTGTGAGGCAAGGAGATTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281710
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281710.1, NP_001268639.1
• RefSeq Size: 6026 bp
• RefSeq ORF: 2193 bp
• Locus ID: 23397
• UniProt ID: Q15003
• Cytogenetics: 2q11.2
• Gene Summary: This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.