BRRN1 (NCAPH) (NM_001281712) Human Untagged Clone

CAT#: SC336957

NCAPH (untagged) - Human non-SMC condensin I complex, subunit H (NCAPH), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCAPH
• Synonyms: BRRN1; CAP-H; CAPH; MCPH23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336957 representing NM_001281712.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCAGAGATTCTTAAACAGAAAGACACCGAACCAACCAACTTTAAAGTGGCTGCGGGTACTCTGGAT
  GCCAGCACCAAGATCTATGCTGTGCGCGTGGATGCCGTCCATGCCGATGTATACAGAGTCCTTGGGGGG
  CTGGGCAAAGATGCACCGTCTTTGGAAGAAGTAGAAGGCCATGTTGCTGATGGAAGTGCTACTGAAATG
  GGAACAACCAAAAAGGCTGTAAAGCCAAAGAAGAAGCACTTACACAGAACTATTGAGCAGAACATAAAC
  AACCTCAATGTCTCCGAAGCAGATCGGAAGTGTGAGATTGATCCCATGTTTCAGAAGACAGCAGCCTCA
  TTTGATGAGTGCAGCACAGCAGGGGTGTTTCTGTCCACTCTCCACTGCCAGGACTACAGAAGTGAACTG
  CTGTTTCCCTCTGATGTCCAGACTCTCTCCACGGGAGAACCTCTCGAGTTGCCAGAGTTAGGTTGTGTA
  GAAATGACAGATTTAAAAGCGCCCTTGCAGCAGTGTGCAGAAGATCGCCAGATCTGCCCTTCCCTGGCC
  GGGTTCCAGTTTACACAGTGGGACAGTGAAACACATAATGAGTCTGTGTCGGCCCTGGTAGACAAGTTT
  AAGAAGAATGACCAGGTATTTGACATCAATGCTGAAGTTGACGAGAGTGACTGTGGAGACTTCCCCGAT
  GGGTCCCTGGGGGATGACTTTGATGCCAACGATGAACCTGACCACACCGCAGTTGGGGATCATGAAGAG
  TTCAGGAGCTGGAAGGAGCCCTGCCAGGTTCAGAGCTGCCAGGAAGAAATGATTTCCCTTGGGGATGGA
  GACATCAGGACCATGTGCCCCCTTCTGTCTATGAAACCTGGAGAATATTCTTATTTCAGTCCTCGGACC
  ATGTCGATGTGGGCTGGCCCGGATCACTGGCGCTTTAGGCCTCGACGCAAACAAGATGCTCCTTCCCAA
  TCAGAAAACAAAAAGAAGAGTACAAAAAAAGATTTTGAAATTGACTTTGAAGATGATATTGACTTTGAT
  GTATATTTTAGAAAAACAAAGGCTGCTACTATTCTGACCAAGTCCACTTTGGAGAACCAGAATTGGAGA
  GCTACCACCCTTCCTACAGATTTCAACTACAATGTTGACACTCTGGTCCAGCTTCACCTCAAACCAGGC
  ACCAGGTTACTTAAGATGGCCCAGGGCCATAGGGTAGAGACTGAGCATTATGAAGAAATTGAAGACTAT
  GATTACAACAACCCTAACGACACCTCCAACTTTTGCCCTGGATTACAGGCTGCTGACAGTGATGATGAA
  GATTTGGATGACTTATTTGTGGGACCTGTTGGGAACTCTGACCTCTCACCTTATCCTTGCCATCCACCT
  AAGACAGCACAACAGAATGGTGACACTCCAGAAGCCCAAGGATTAGACATCACAACATATGGGGAGTCA
  AACTTGGTAGCTGAGCCTCAGAAGGTAAATAAAATTGAAATTCACTATGCCAAGACTGCCAAAAAGATG
  GACATGAAGAAACTGAAGCAGAGCATGTGGAGTCTGCTGACAGCGCTCTCCGGAAAGGAGGCAGATGCA
  GAGGCAAACCACAGGGAAGCTGGAAAAGAAGCGGCCCTGGCAGAAGTGGCTGACGAGAAGATGCTTAGC
  GGGCTCACGAAGGACCTGCAGAGGAGCCTGCCCCCTGTCATGGCTCAGAACCTCTCCATACCTCTGGCT
  TTTGCCTGTCTCCTACATTTAGCCAATGAAAAGAATCTAAAACTGGAAGGAACAGAGGACCTCTCTGAT
  GTTCTTGTGAGGCAAGGAGATTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281712
• Insert Size: 1818 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281712.1
• RefSeq Size: 5806 bp
• RefSeq ORF: 1818 bp
• Locus ID: 23397
• UniProt ID: Q15003
• Cytogenetics: 2q11.2
• MW: 67.5 kDa
• Gene Summary: This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (4) lacks an alternate exon in the coding region and initiates translation at an alternate start codon compared to variant 1. The resulting protein (isoform 4) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.