ABLIM3 (NM_001301027) Human Untagged Clone

CAT#: SC336868

ABLIM3 (untagged) - Human actin binding LIM protein family, member 3 (ABLIM3), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABLIM3
• Synonyms: HMFN1661
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336868 representing NM_001301027.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCATCCAGTGCTACCGCTGTGGAGACACCTGCAAAGGGGAAGTGGTCCGCGTGCACAACAACCACT
  TCCACATCAGATGCTTCACCTGTCAAGTATGTGGCTGTGGCCTGGCCCAGTCAGGCTTCTTCTTCAAGA
  ACCAGGAGTACATCTGCACCCAGGACTACCAGCAACTCTATGGCACCCGCTGTGACAGCTGCCGGGACT
  TCATCACAGGCGAAGTCATCTCGGCCCTGGGCCGCACTTACCACCCCAAGTGCTTCGTGTGCAGCTTGT
  GCAGGACATCTCCAGGGATGGAGAGCTCATCCCTGCCTCCTGCTTTACCTTCCGCATCACCATCACTTG
  TTAATTCAGCAAGTGAAGCCTTTCCCCATTGGAGACAAGGTGACCTTCAGCGGTAAAGAATGTGTGTGC
  CAAACGTGCTCCCAGTCCATGGCCAGCAGTAAGCCCATCAAGATTCGTGGACCAAGCCACTGTGCCGGG
  TGCAAGGAGGAGATCAAGCACGGCCAGTCACTCCTGGCTCTGGACAAGCAGTGGCACGTCAGCTGCTTC
  AAGTGCCAGACCTGCAGCGTCATCCTCACCGGGGAGTATATCAGCAAGGATGGTGTTCCATACTGTGAG
  TCCGACTACCATGCCCAGTTTGGCATTAAATGTGAGACTTGTGACCGATACATCAGTGGCAGAGTCTTG
  GAGGCAGGAGGGAAGCACTACCACCCAACCTGTGCCAGGTGTGTACGCTGCCACCAGATGTTCACCGAA
  GGAGAGGAAATGTACCTCACAGGTTCCGAGGTTTGGCACCCCATCTGCAAACAGGCAGCCCGGGCAGAG
  AAGAAGTTAAAGCATAGACGGACATCTGAAACCTCCATCTCACCCCCTGGATCCAGCATTGGGTCACCC
  AACCGAGTCATCTGCGACATCTACGAGAACCTGGACCTCCGGCAGAGACGGGCCTCCAGCCCGGGGTAC
  ATAGACTCCCCCACCTACAGCCGGCAGGGCATGTCCCCCACCTTCTCCCGCTCACCTCACCACTACTAC
  CGCTCTGGTGATTTGTCTACAGCAACCAAGAGCAAAACAAGTGAAGACATCAGCCAGACCTCCAAGTAC
  AGTCCCATCTACTCGCCAGACCCCTACTATGCTTCGGAGTCTGAGTACTGGACCTACCATGGGTCCCCC
  AAAGTGCCCCGAGCCAGAAGGTTCTCGTCTGGAGGAGAGGAGGATGATTTTGACCGCAGCATGCACAAG
  CTCCAAAGTGGAATTGGCCGGCTGATTCTGAAGGAAGAAATGAAGGCCCGGTCGAGCTCCTATGCAGAT
  CCCTGGACCCCTCCCCGGAGCTCCACCAGCAGCCGGGAAGCCCTGCACACAGCTGGCTATGAGATGTCC
  CTCAATGGCTCCCCTCGGTCGCACTACCTGGCTGACAGTGATCCTCTCATCTCCAAATCTGCCTCCCTG
  CCTGCCTACCGAAGAAATGGGCTGCACAGGACACCCAGCGCAGACCTCTTCCACTACGACAGCATGAAC
  GCAGTCAACTGGGGCATGCGAGAGTACAAGATCTACCCTTATGAACTGCTGCTGGTGACTACAAGAGGA
  AGAAACCGACTGCCCAAGGATGTAGACAGGACCCGTTTAGAGCGCCACCTGTCCCAGGAAGAGTTCTAC
  CAAGTCTTTGGCATGACCATCTCTGAGTTTGACCGGCTGGCCCTCTGGAAGAGGAATGAACTGAAGAAG
  CAAGCCCGGCTGTTCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301027
• Insert Size: 1743 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301027.1
• RefSeq Size: 4459 bp
• RefSeq ORF: 1743 bp
• Locus ID: 22885
• UniProt ID: O94929
• Cytogenetics: 5q32
• Protein Pathways: Axon guidance
• MW: 64.8 kDa
• Gene Summary: This gene encodes a member of the actin-binding LIM (abLIM) family of proteins. These proteins are characterized by an N-terminal LIM domain and a C-terminal dematin-like domain. The encoded protein interacts with actin filaments and may be a component of adherens junctions in several cell types. A variant of this gene may be associated with pain sensitivity in male human patients. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (4) has an additional exon in the 5' region, which results in translation initiation at a downstream start codon, and lacks several in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (3) has a distinct N-terminus and lacks two internal segments, compared to isoform 1.