ABLIM3 (NM_001301027) Human Tagged ORF Clone

CAT#: RG238974

ABLIM3 (tGFP-tagged) - Human actin binding LIM protein family, member 3 (ABLIM3), transcript variant 4

Product Images

Circular map for RG238974

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ABLIM3
• Synonyms: HMFN1661
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238974 representing NM_001301027.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCATCCAGTGCTACCGCTGTGGAGACACCTGCAAAGGGGAAGTGGTCCGCGTGCACAACAACCACT
  TCCACATCAGATGCTTCACCTGTCAAGTATGTGGCTGTGGCCTGGCCCAGTCAGGCTTCTTCTTCAAGA
  ACCAGGAGTACATCTGCACCCAGGACTACCAGCAACTCTATGGCACCCGCTGTGACAGCTGCCGGGACT
  TCATCACAGGCGAAGTCATCTCGGCCCTGGGCCGCACTTACCACCCCAAGTGCTTCGTGTGCAGCTTGT
  GCAGGACATCTCCAGGGATGGAGAGCTCATCCCTGCCTCCTGCTTTACCTTCCGCATCACCATCACTTG
  TTAATTCAGCAAGTGAAGCCTTTCCCCATTGGAGACAAGGTGACCTTCAGCGGTAAAGAATGTGTGTGC
  CAAACGTGCTCCCAGTCCATGGCCAGCAGTAAGCCCATCAAGATTCGTGGACCAAGCCACTGTGCCGGG
  TGCAAGGAGGAGATCAAGCACGGCCAGTCACTCCTGGCTCTGGACAAGCAGTGGCACGTCAGCTGCTTC
  AAGTGCCAGACCTGCAGCGTCATCCTCACCGGGGAGTATATCAGCAAGGATGGTGTTCCATACTGTGAG
  TCCGACTACCATGCCCAGTTTGGCATTAAATGTGAGACTTGTGACCGATACATCAGTGGCAGAGTCTTG
  GAGGCAGGAGGGAAGCACTACCACCCAACCTGTGCCAGGTGTGTACGCTGCCACCAGATGTTCACCGAA
  GGAGAGGAAATGTACCTCACAGGTTCCGAGGTTTGGCACCCCATCTGCAAACAGGCAGCCCGGGCAGAG
  AAGAAGTTAAAGCATAGACGGACATCTGAAACCTCCATCTCACCCCCTGGATCCAGCATTGGGTCACCC
  AACCGAGTCATCTGCGACATCTACGAGAACCTGGACCTCCGGCAGAGACGGGCCTCCAGCCCGGGGTAC
  ATAGACTCCCCCACCTACAGCCGGCAGGGCATGTCCCCCACCTTCTCCCGCTCACCTCACCACTACTAC
  CGCTCTGGTGATTTGTCTACAGCAACCAAGAGCAAAACAAGTGAAGACATCAGCCAGACCTCCAAGTAC
  AGTCCCATCTACTCGCCAGACCCCTACTATGCTTCGGAGTCTGAGTACTGGACCTACCATGGGTCCCCC
  AAAGTGCCCCGAGCCAGAAGGTTCTCGTCTGGAGGAGAGGAGGATGATTTTGACCGCAGCATGCACAAG
  CTCCAAAGTGGAATTGGCCGGCTGATTCTGAAGGAAGAAATGAAGGCCCGGTCGAGCTCCTATGCAGAT
  CCCTGGACCCCTCCCCGGAGCTCCACCAGCAGCCGGGAAGCCCTGCACACAGCTGGCTATGAGATGTCC
  CTCAATGGCTCCCCTCGGTCGCACTACCTGGCTGACAGTGATCCTCTCATCTCCAAATCTGCCTCCCTG
  CCTGCCTACCGAAGAAATGGGCTGCACAGGACACCCAGCGCAGACCTCTTCCACTACGACAGCATGAAC
  GCAGTCAACTGGGGCATGCGAGAGTACAAGATCTACCCTTATGAACTGCTGCTGGTGACTACAAGAGGA
  AGAAACCGACTGCCCAAGGATGTAGACAGGACCCGTTTAGAGCGCCACCTGTCCCAGGAAGAGTTCTAC
  CAAGTCTTTGGCATGACCATCTCTGAGTTTGACCGGCTGGCCCTCTGGAAGAGGAATGAACTGAAGAAG
  CAAGCCCGGCTGTTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238974
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSSSATAVETPAKGKWSACTTTTSTSDASPVKYVAVAWPSQASSSRTRSTSAPRTTSNSMAPAVTAAGT
  SSQAKSSRPWAALTTPSASCAACAGHLQGWRAHPCLLLYLPHHHHLLIQQVKPFPIGDKVTFSGKECVC
  QTCSQSMASSKPIKIRGPSHCAGCKEEIKHGQSLLALDKQWHVSCFKCQTCSVILTGEYISKDGVPYCE
  SDYHAQFGIKCETCDRYISGRVLEAGGKHYHPTCARCVRCHQMFTEGEEMYLTGSEVWHPICKQAARAE
  KKLKHRRTSETSISPPGSSIGSPNRVICDIYENLDLRQRRASSPGYIDSPTYSRQGMSPTFSRSPHHYY
  RSGDLSTATKSKTSEDISQTSKYSPIYSPDPYYASESEYWTYHGSPKVPRARRFSSGGEEDDFDRSMHK
  LQSGIGRLILKEEMKARSSSYADPWTPPRSSTSSREALHTAGYEMSLNGSPRSHYLADSDPLISKSASL
  PAYRRNGLHRTPSADLFHYDSMNAVNWGMREYKIYPYELLLVTTRGRNRLPKDVDRTRLERHLSQEEFY
  QVFGMTISEFDRLALWKRNELKKQARLF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301027
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001301027.2
• RefSeq Size: 4459 bp
• RefSeq ORF: 1743 bp
• Locus ID: 22885
• UniProt ID: O94929
• Cytogenetics: 5q32
• Protein Pathways: Axon guidance
• MW: 65.2 kDa
• Gene Summary: This gene encodes a member of the actin-binding LIM (abLIM) family of proteins. These proteins are characterized by an N-terminal LIM domain and a C-terminal dematin-like domain. The encoded protein interacts with actin filaments and may be a component of adherens junctions in several cell types. A variant of this gene may be associated with pain sensitivity in male human patients. [provided by RefSeq, Sep 2016]