OTOP3 (NM_001272005) Human Untagged Clone

CAT#: SC336863

OTOP3 (untagged) - Human otopetrin 3 (OTOP3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OTOP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336863 representing NM_001272005.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTCTCCCGGCCTCAGCCCCTGCTGAGGCTACACCCATGCCTTCTTCAGAAGCACAGGAGACTGAA
  GCAGCCCCGGAGAAGGAGAACCGAGTGGATGTGGGGGCCGAGGAGAGAGCGGCCGCCACCCGGCCCCGG
  CAGAAGTCCTGGCTGGTGAGGCATTTCTCTCTGCTGCTGCGGCGGGACCGGCAGGCCCAGAAGGCTGGA
  CAACTCTTCTCGGGGCTCCTGGCCCTGAATGTGGTGTTCCTGGGTGGCGCCTTCATCTGCAGCATGATC
  TTCAACAAGGTGGCCGTCACTCTGGGTGACGTGTGGATCCTGCTGGCCACGCTGAAGGTCCTCTCCCTG
  CTTTGGCTTCTCTACTATGTGGCAAGCACCACCCGCCGACCACACGCCGTGCTCTACCAAGATCCCCAC
  GCGGGGCCCCTCTGGGTGCGGGGTTCCCTAGTGCTCTTCGGCAGCTGCACCTTCTGCCTCAACATCTTC
  CGAGTGGGCTACGATGTGAGCCACATCCGCTGCAAGTCACAGCTGGACCTTGTCTTCTCTGTCATCGAG
  ATGGTCTTCATCGGCGTCCAGACCTGGGTGCTCTGGAAACACTGCAAAGACTGTGTTCGGGTCCAGACC
  AACTTCACTAGGTGTGGCCTGATGCTGACCCTGGCCACAAACCTGCTGCTGTGGGTTCTGGCCGTTACC
  AATGACTCCATGCACCGAGAGATCGAAGCTGAGCTTGGCATCCTCATGGAAAAATCCACAGGCAATGAG
  ACCAACACCTGTCTGTGCCTCAATGCCACCGCGTGTGAAGCTTTCCGGAGAGGCTTCCTGATGCTCTAC
  CCCTTCAGCACTGAGTACTGCCTCATCTGCTGTGCTGTGCTGTTTGTCATGTGGAAGAACGTGGGCCGC
  CACGTGGCACCCCACATGGGTGCCCACCCTGCCACCGCACCCTTCCACCTGCACGGGGCCATCTTCGGG
  CCGCTGCTGGGCCTGCTGGTGCTGCTGGCAGGTGTGTGCGTCTTTGTGCTCTTCCAAATCGAGGCCAGT
  GGCCCTGCCATTGCTTGCCAGTACTTCACCCTCTACTATGCCTTCTATGTGGCTGTGCTGCCCACCATG
  AGTCTGGCGTGCCTGGCGGGCACAGCCATACACGGGCTGGAGGAGAGAGAGCTGGACACGGTCAAGAAC
  CCTACCCGCAGCCTGGATGTGGTGCTGCTAATGGGTGCTGCACTGGGCCAGATGGGCATCGCCTATTTC
  TCCATCGTGGCCATTGTGGCCAAGCGCCCGCATGAGCTGCTCAACCGCCTCATCCTGGCCTACTCGCTG
  CTGCTCATCCTGCAGCACATCGCTCAGAACCTCTTCATCATCGAGGGCCTGCACCGGCGCCCACTCTGG
  GAGACAGTTCCCGAGGGCCTGGCAGGAAAGCAGGAGGCTGAGCCTCCCCGCAGAGGCTCCTTGCTGGAG
  CTGGGCCAGGGCCTGCAGCGGGCCTCACTGGCCTACATCCACTCCTACAGCCACCTCAACTGGAAGCGG
  AGGGCACTCAAGGAGATCTCACTCTTCCTCATCCTCTGCAATATCACACTGTGGATGATGCCTGCATTT
  GGCATACACCCGGAGTTTGAGAACGGGCTAGAAAAGGATTTCTACGGCTACCAGATATGGTTCGCCATC
  GTCAACTTCGGCCTGCCTCTGGGGGTCTTCTACCGCATGCACTCTGTGGGAGGCCTGGTGGAGGTCTAC
  CTGGGGGCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001272005
• Insert Size: 1737 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272005.1
• RefSeq Size: 2433 bp
• RefSeq ORF: 1737 bp
• Locus ID: 347741
• UniProt ID: Q7RTS5
• Cytogenetics: 17q25.1
• Protein Families: Transmembrane
• MW: 64.4 kDa
• Gene Summary: Proton-selective channel that specifically transports protons into cells. Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses alternate splice sites at the end of two exons compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.