Nicastrin (NCSTN) (NM_001290186) Human Untagged Clone

CAT#: SC336829

NCSTN (untagged) - Human nicastrin (NCSTN), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCSTN
• Synonyms: ATAG1874
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336829 representing NM_001290186.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTACGGCAGGGGGTGGCTCTGGGGCTGACCCGGGAAGTCGGGGTCTCCTTCGCCTTCTGTCTTTC
  TGCGTCCTACTAGCAGGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATATATATCCCCTTAAATAAA
  ACAGCTCCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATTGGCTGCCAGTCTTCAATTAGTGGAGAC
  ACAGGGGTTATCCACGTAGTAGAGAAAGAGGAGGACCTACAGTGGGTATTGACTGATGGCCCCAACCCC
  CCTTACATGGTTCTGCTGGAGAGCAAGCATTTTACCAGGGATTTAATGGAGAAGCTGAAAGGGAGAACC
  AGCCGAATTGCTGGTCTTGCAGTGTCCTTGACCAAGCCCAGTCCTGCCTCAGGCTTCTCTCCTAGTGTA
  CAGTGCCCAAATGATGGGTTTGGTGTTTACTCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGAGAA
  ATACAGTGGAATTCGCTGGGCAATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATCTTTCTTCTTGAA
  GATGAAAATGAAACCAAAGTCATCAAGCAGGAAACTTTTGACTACATTGGCAGCTCGAGGATGGTCTAC
  GATATGGAGAAGGGCAAGTTTCCCGTGCAGTTAGAGAATGTTGACTCATTTGTGGAGCTGGGACAGGTG
  GCCTTAAGAACTTCATTAGAGCTTTGGATGCACACAGATCCTGTTTCTCAGAAAAATGAGTCTGTACGG
  AACCAGGTGGAGGATCTCCTGGCCACATTGGAGAAGAGTGGTGCTGGTGTCCCTGCTGTCATCCTCAGG
  AGGCCAAATCAGTCCCAGCCTCTCCCACCATCTTCCCTGCAGCGATTTCTTCGAGCTCGAAACATCTCT
  GGCGTTGTTCTGGCTGACCACTCTGGTGCCTTCCATAACAAATATTACCAGAGTATTTACGACACTGCT
  GAGAACATTAATGTGAGCTATCCCGAATGGCTGAGCCCTGAAGAGGACCTGAACTTTGTAACAGACACT
  GCCAAGGCCCTGGCAGATGTGGCCACGGTGCTGGGACGTGCTCTGTATGAGCTTGCAGGAGGAACCAAC
  TTCAGCGACACAGTTCAGGCTGATCCCCAAACGGTTACCCGCCTGCTCTATGGGTTCCTGATTAAAGCC
  AACAACTCATGGTTCCAGTCTATCCTCAGGCAGGACCTAAGGTCCTACTTGGGTGACGGGCCTCTTCAA
  CATTACATCGCTGTCTCCAGCCCCACCAACACCACTTATGTTGTACAGTATGCCTTGGCAAATTTGACT
  GGCACAGTGGTCAACCTCACCCGAGAGCAGTGCCAGGATCCAAGTAAAGTCCCAAGTGAAAACAAGGAT
  CTGTATGAGTACTCATGGGTCCAGGGCCCTTTGCATTCTAATGAGACGGACCGACTCCCCCGGTGTGTG
  CGTTCTACTGCACGATTAGCCAGGGCCTTGTCTCCTGCCTTTGAACTGAGTCAGTGGAGCTCTACTGAA
  TACTCTACATGGACTGAGAGCCGCTGGAAAGATATCCGTGCCCGGATATTTCTCATCGCCAGCAAAGAG
  CTTGAGTTGATCACCCTGACAGTGGGCTTCGGCATCCTCATCTTCTCCCTCATCGTCACCTACTGCATC
  AATGCCAAAGCTGATGTCCTTTTCATTGCTCCCCGGGAGCCAGGAGCTGTGTCATACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290186
• Insert Size: 1716 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290186.1
• RefSeq Size: 2530 bp
• RefSeq ORF: 1716 bp
• Locus ID: 23385
• UniProt ID: Q92542
• Cytogenetics: 1q23.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Notch signaling pathway
• MW: 63.3 kDa
• Gene Summary: This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (3) lacks three alternate in-frame exons in the coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1.