CDC2L6 (CDK19) (NM_001300960) Human Untagged Clone

CAT#: SC336240

CDK19 (untagged) - Human cyclin-dependent kinase 19 (CDK19), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC2L6
• Synonyms: bA346C16.3; CDC2L6; CDK11; DEE87; EIEE87
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336240 representing NM_001300960.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATTATGATTTCAAGGCGAAGCTGGCGGCGGAGCGGGAGCGGGTGGAGGATTTGTTTGAGTACGAA
  GGGTGCAAAGTGGGACGCGGCACCTACGGTCACGTCTACAAGGCGAGGCGGAAAGATGGAAAAGATGAA
  AAGGAATATGCATTGAAGCAAATTGAAGGCACAGGAATATCCATGTCGGCTTGTAGAGAGATTGCACTT
  TTGCGAGAATTGAAGCACCCTAATGTGATTGCATTGCAGAAGGTGTTCCTTTCTCACAGTGACAGGAAG
  GTATGGCTGCTGTTTGATTATGCAGAGCATGACTTGTGGCATATTATTAAGTTTCACCGTGCATCAAAA
  GCAAATAAAAAGCCCATGCAGTTGCCAAGATCTATGGTTAAATCCTTACTTTACCAGATTCTTGATGGT
  ATCCATTACCTCCATGCAAATTGGGTGCTTCACAGAGACTTGAAACCAGCAAATATCCTAGTAATGGGA
  GAAGGTCCTGAGAGGGGGAGAGTCAAAATAGATATATGGGCAATAGGTTGTATATTTGCTGAATTGTTG
  ACTTCGGAACCTATTTTTCACTGTCGTCAGGAAGATATAAAAACAAGCAATCCCTTTCATCATGATCAA
  CTGGATCGGATATTTAGTGTCATGGGGTTTCCTGCAGATAAAGACTGGGAAGATATTAGAAAGATGCCA
  GAATATCCCACACTTCAAAAAGACTTTAGAAGAACAACGTATGCCAACAGTAGCCTCATAAAGTACATG
  GAGAAACACAAGGTCAAGCCTGACAGCAAAGTGTTCCTCTTGCTTCAGAAACTCCTGACCATGGATCCA
  ACCAAGAGAATTACCTCGGAGCAAGCTCTGCAGGATCCCTATTTTCAGGAGGACCCTTTGCCAACATTA
  GATGTATTTGCCGGCTGCCAGATTCCATACCCCAAACGAGAATTCCTTAATGAAGATGATCCTGAAGAA
  AAAGGTGACAAGAATCAGCAACAGCAGCAGAACCAGCATCAGCAGCCCACAGCCCCTCCACAGCAGGCA
  GCAGCCCCTCCACAGGCGCCCCCACCACAGCAGAACAGCACCCAGACCAACGGGACCGCAGGTGGGGCT
  GGGGCCGGGGTCGGGGGCACCGGAGCAGGGTTGCAGCACAGCCAGGACTCCAGCCTGAACCAGGTGCCT
  CCAAACAAGAAGCCACGGCTAGGGCCTTCAGGCGCAAACTCAGGTGGACCTGTGATGCCCTCGGATTAT
  CAGCACTCCAGTTCTCGCCTGAATTACCAAAGCAGCGTTCAGGGATCCTCTCAGTCCCAGAGCACACTT
  GGCTACTCTTCCTCGTCTCAGCAGAGCTCACAGTACCACCCATCTCACCAGGCCCACCGGTACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001300960
• Insert Size: 1377 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300960.1
• RefSeq Size: 6114 bp
• RefSeq ORF: 1377 bp
• Locus ID: 23097
• UniProt ID: Q9BWU1
• Cytogenetics: 6q21
• Protein Families: Druggable Genome, Protein Kinase
• MW: 51.8 kDa
• Gene Summary: This gene encodes a protein that is one of the components of the Mediator co-activator complex. The Mediator complex is a multi-protein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.