MEK4 (MAP2K4) (NM_001281435) Human Untagged Clone

CAT#: SC335938

MAP2K4 (untagged) - Human mitogen-activated protein kinase kinase 4 (MAP2K4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEK4
• Synonyms: JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335938 representing NM_001281435.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCTCCGAGCCCGAGCGGCGGCGGCGGCTCCGGGGGCGGCAGCGGCAGCGGCACCCCCGGCCCC
  GTAGGGTCCCCGGCGCCAGGCCACCCGGCCGTCAGCAGCATGCAGGGCTTTCAGATAAACTTCTGTGAA
  AAGGCACAAAGTAAACGCAAAGCACTGAAGTTGAATTTTGCAAATCCACCTTTCAAATCTACAGCAAGG
  TTTACTCTGAATCCCAATCCTACAGGAGTTCAAAACCCACACATAGAGAGACTGAGAACACACAGCATT
  GAGTCATCAGGAAAACTGAAGATCTCCCCTGAACAACACTGGGATTTCACTGCAGAGGACTTGAAAGAC
  CTTGGAGAAATTGGACGAGGAGCTTATGGTTCTGTCAACAAAATGGTCCACAAACCAAGTGGGCAAATA
  ATGGCAGTTAAAAGAATTCGGTCAACAGTGGATGAAAAAGAACAAAAACAACTTCTTATGGATTTGGAT
  GTAGTAATGCGGAGTAGTGATTGCCCATACATTGTTCAGTTTTATGGTGCACTCTTCAGAGAGGGTGAC
  TGTTGGATCTGTATGGAACTCATGTCTACCTCGTTTGATAAGTTTTACAAATATGTATATAGTGTATTA
  GATGATGTTATTCCAGAAGAAATTTTAGGCAAAATCACTTTAGCAACTGTGAAAGCACTAAACCACTTA
  AAAGAAAACTTGAAAATTATTCACAGAGATATCAAACCTTCCAATATTCTTCTGGACAGAAGTGGAAAT
  ATTAAGCTCTGTGACTTCGGCATCAGTGGACAGCTTGTGGACTCTATTGCCAAGACAAGAGATGCTGGC
  TGTAGGCCATACATGGCACCTGAAAGAATAGACCCAAGCGCATCACGACAAGGATATGATGTCCGCTCT
  GATGTCTGGAGTTTGGGGATCACATTGTATGAGTTGGCCACAGGCCGATTTCCTTATCCAAAGTGGAAT
  AGTGTATTTGATCAACTAACACAAGTCGTGAAAGGAGATCCTCCGCAGCTGAGTAATTCTGAGGAAAGG
  GAATTCTCCCCGAGTTTCATCAACTTTGTCAACTTGTGCCTTACGAAGGATGAATCCAAAAGGCCAAAG
  TATAAAGAGCTTCTGAAACATCCCTTTATTTTGATGTATGAAGAACGTGCCGTTGAGGTCGCATGCTAT
  GTTTGTAAAATCCTGGATCAAATGCCAGCTACTCCCAGCTCTCCCATGTATGTCGATTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281435
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281435.1
• RefSeq Size: 3873 bp
• RefSeq ORF: 1233 bp
• Locus ID: 6416
• UniProt ID: P45985
• Cytogenetics: 17p12
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway
• MW: 45.6 kDa
• Gene Summary: This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is longer compared to isoform 1.