UGT (UGT2B4) (NM_001297615) Human Untagged Clone

CAT#: SC335668

UGT2B4 (untagged) - Human UDP glucuronosyltransferase 2 family, polypeptide B4 (UGT2B4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UGT
• Synonyms: HLUG25; UDPGT2B4; UDPGTh-1; UDPGTH1; UGT2B11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335668 representing NM_001297615.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTATGAAATGGACTTCAGCTCTTCTGCTGATACAGCTGAGCTGTTACTTTAGCTCTGGGAGTTGT
  GGAAAGGTGCTGGTGTGGCCCACAGAATTCAGCCACTGGATGAATATAAAGACAATCCTGGATGAACTT
  GTCCAGAGAGGTCATGAGGTGACTGTATTGGCATCTTCAGCTTCCATTTCTTTCGATCCCAACAGCCCA
  TCTACTCTTAAATTTGAAGTTTATCCTGTATCTTTAACTAAAACTGAGTTTGAGGATATTATCAAGCAG
  CTGGTTAAGAGATGGGCAGAACTTCCAAAAGACACATTTTGGTCATATTTTTCACAAGTACAAGAAATC
  ATGTGGACATTTAATGACATACTTAGAAAGTTCTGTAAGGATATAGTTTCAAATAAGAAACTTATGAAG
  AAACTACAGGAGTCAAGATTTGATGTTGTTCTTGCAGATGCTGTTTTCCCCTTTGGTGAGCTGCTGGCC
  GAGTTACTTAAAATACCCTTTGTCTACAGCCTCCGCTTCTCTCCTGGCTACGCAATTGAAAAGCATAGT
  GGAGGACTTCTGTTCCCTCCTTCCTATGTGCCTGTTGTTATGTCAGAACTAAGTGACCAAATGACTTTC
  ATAGAGAGGGTAAAAAATATGATCTATGTGCTTTATTTTGAATTTTGGTTCCAAATATTTGACATGAAG
  AAGTGGGATCAGTTCTACAGTGAAGTTCTAGGAAGACCCACTACGTTATCTGAGACAATGGCAAAAGCT
  GACATATGGCTTATTCGAAACTACTGGGATTTTCAATTTCCTCACCCACTCTTACCAAATGTTGAGTTC
  GTTGGAGGACTCCACTGCAAACCTGCCAAACCCCTACCGAAGGAAATGGAAGAGTTTGTCCAGAGCTCT
  GGAGAAAATGGTGTTGTGGTGTTTTCTCTGGGGTCGATGGTCAGTAACACGTCAGAAGAAAGGGCCAAT
  GTAATTGCATCAGCCCTTGCCAAGATCCCACAAAAGGTTCTGTGGAGATTTGATGGGAATAAACCAGAT
  ACTTTAGGACTCAATACTCGGCTGTACAAGTGGATACCCCAGAATGATCTTCTTGATATAAAGAGAATG
  CTATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001297615
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297615.1
• RefSeq Size: 1899 bp
• RefSeq ORF: 1110 bp
• Locus ID: 7363
• UniProt ID: P06133
• Cytogenetics: 4q13.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• MW: 42.7 kDa
• Gene Summary: UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.