Adenylate cyclase 1 (ADCY1) (NM_001281768) Human Untagged Clone

CAT#: SC335456

ADCY1 (untagged) - Human adenylate cyclase 1 (brain) (ADCY1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Adenylate cyclase 1
• Synonyms: AC1; DFNB44
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335456 representing NM_001281768.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTATGGGGTCTTTGTGCGGATTCTGACTGAGCGTTCACAGAGGAAGGCGTTCCTGCAGGCCCGGAGC
  TGCATTGAGGACCGACTGAGGCTGGAGGATGAGAACGAGAAGCAGGAGCGGCTCCTCATGAGCCTCCTG
  CCCCGGAACGTTGCCATGGAGATGAAGGAGGACTTCCTGAAGCCCCCTGAGAGGATTTTCCACAAGATT
  TACATCCAGAGGCACGACAATGTGAGCATCCTGTTTGCTGACATCGTGGGTTTCACGGGCTTGGCATCC
  CAGTGCACAGCCCAGGAGCTGGTGAAACTCCTCAATGAGCTCTTCGGCAAGTTCGATGAATTAGCCACG
  GAGAACCACTGTCGCCGCATCAAGATTCTCGGGGACTGCTACTACTGCGTGTCGGGCCTCACCCAGCCC
  AAGACTGACCATGCCCACTGCTGTGTGGAGATGGGACTCGACATGATTGATACCATCACATCTGTGGCT
  GAAGCCACCGAGGTGGATCTGAACATGCGTGTGGGTCTGCACACGGGCAGGGTCCTCTGTGGTGTCCTG
  GGCTTGCGCAAGTGGCAGTACGACGTGTGGTCCAATGATGTGACCTTGGCCAATGTCATGGAAGCCGCT
  GGCCTGCCAGGGAAGGTTCATATCACAAAGACGACCCTAGCGTGCTTGAATGGGGACTACGAGGTAGAA
  CCGGGTTACGGACATGAGAGGAACAGTTTCTTGAAAACTCATAACATCGAAACCTTTTTTATTGTGCCA
  TCCCATCGCCGAAAGATATTTCCAGGCCTGATTCTCTCAGATATAAAACCGGCCAAAAGGATGAAGTTC
  AAGACTGTCTGCTACCTGCTGGTGCAGCTCATGCACTGCCGGAAAATGTTCAAGGCCGAGATCCCCTTC
  TCCAATGTCATGACCTGCGAGGACGATGACAAGGTTCAGATCCATCCCTCACTGAGTGGCCTGGATGTC
  CTCCTGGGGTTTTGGTCTCCCACCCTGCAACGGGGACGACTGTGCACGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281768
• Insert Size: 1017 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281768.1
• RefSeq Size: 1763 bp
• RefSeq ORF: 1017 bp
• Locus ID: 107
• Cytogenetics: 7p12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
• MW: 38.7 kDa
• Gene Summary: This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) differs in the 5' and 3' UTR and has multiple differences in the coding region compared to variant 1. The encoded isoform (2) has a shorter N-terminus and a distinct C-terminus, compared to isoform 1.