EFTUD2 (NM_001258354) Human Untagged Clone

CAT#: SC332669

EFTUD2 (untagged) - Homo sapiens elongation factor Tu GTP binding domain containing 2 (EFTUD2), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EFTUD2
• Synonyms: MFDGA; MFDM; SNRNP116; Snrp116; Snu114; U5-116KD
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332669 representing NM_001258354.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGATACCGACTTATATGATGAGTTTGGGAATTATATTGGACCAGAGCTTGATTCTGATGAAGATGAT
  GATGAATTGGGTAGAGAGACCAAAGATCTTGATGAGATGGATGATGATGACGACGACGATGACGTAGGA
  GATCATGACGATGACCACCCTGGGATGGAGGTGGTGCTGCATGAGGACAAGAAGTACTACCCAACAGCC
  GAGGAGGTGTATGGTCCTGAGGTGGAGACCATAGTTCAAGAGGAAGACACTCAGCCTCTCACAGAACCC
  ATTATTAAGCCAGTGAAAACCAAGAAATTCACTCTGATGGAGCAGACATTACCTGTTACGGTGTATGAG
  ATGGATTTCTTGGCGGATCTGATGGATAACTCAGAGCTCATCAGAAATGTGACCCTTTGTGGACATCTC
  CACCATGGCAAGACTCACCCGGAAATCAGAAAGCGCTATGACCAAGATCTGTGCTATACTGACATCCTC
  TTCACAGAGCAAGAGAGAGGTGTAGGCATCAAAAGCACTCCTGTGACAGTGGTCTTGCCAGACACCAAA
  GGAAAATCTTATCTCTTCAATATCATGGACACTCCAGGACATGTGAATTTCTCTGATGAGGTCACAGCT
  GGCTTGCGCATCTCAGATGGAGTGGTCCTTTTCATTGATGCTGCTGAGGGGGTGATGCTGAACACAGAG
  CGGCTGATCAAGCATGCGGTGCAGGAGAGGCTGGCAGTCACTGTGTGCATCAACAAGATTGACCGGCTG
  ATCCTGGAGCTGAAGCTGCCTCCAACTGATGCTTATTACAAGCTGCGCCACATTGTGGATGAGGTCAAT
  GGATTAATAAGCATGTATTCCACTGATGAGAACCTGATCCTTTCCCCACTCCTGGGTAACGTCTGCTTC
  TCCAGCTCCCAGTACAGCATCTGCTTCACGCTGGGCTCCTTTGCCAAGATCTATGCCGACACCTTTGGT
  GACATTAATTACCAAGAATTTGCTAAAAGACTCTGGGGTGACATCTACTTCAACCCTAAGACGCGAAAG
  TTCACCAAAAAGGCCCCAACTAGCAGCTCCCAGAGAAGTTTCGTGGAGTTTATCTTGGAGCCTCTTTAT
  AAGATCCTCGCCCAGGTTGTAGGTGACGTGGACACCAGCCTCCCACGGACCCTAGACGAGCTTGGCATC
  CACCTGACGAAGGAGGAGCTGAAGCTGAACATCCGCCCCTTGCTCAGGCTGGTCTGCAAAAAGTTCTTT
  GGCGAGTTCACAGGCTTTGTGGACATGTGTGTGCAGCATATCCCTTCTCCAAAGGTGGGCGCCAAGCCC
  AAGATTGAGCACACCTACACCGGTGGTGTGGACTCCGACCTCGGCGAGGCTATGAGTGACTGTGACCCT
  GATGGCCCCCTGATGTGCCACACTACTAAGATGTACAGCACAGATGATGGAGTCCAGTTTCACGCCTTT
  GGCCGGGTGCTGAGTGGCACCATTCATGCTGGGCAGCCTGTGAAGGTACTGGGGGAGAACTACACCCTG
  GAGGATGAGGAAGACTCCCAGATATGCACCGTGGGCCGCCTTTGGATCTCTGTGGCCAGGTACCACATC
  GAGGTGAACCGTGTTCCTGCTGGCAACTGGGTTCTGATTGAAGGTGTTGATCAACCAATTGTGAAGACA
  GCAACCATAACCGAACCCCGAGGCAATGAGGAGGCTCAGATTTTCCGACCCTTGAAGTTCAATACCACA
  TCTGTTATCAAGATTGCTGTGGAGCCAGTCAACCCCTCAGAGCTGCCCAAGATGCTTGATGGCCTGCGC
  AAGGTCAACAAGAGCTATCCATCCCTCACCACCAAGGTGGAGGAGTCTGGCGAGCATGTGATCCTGGGC
  ACTGGGGAGCTCTACCTGGACTGTGTGATGCATGATTTGCGGAAGATGTACTCAGAGATAGACATCAAG
  GTGGCTGACCCAGTTGTCACGTTTTGTGAGACGGTGGTGGAAACATCCTCCCTCAAGTGCTTTGCTGAA
  ACGCCTAATAAGAAGAACAAGATCACCATGATTGCTGAGCCTCTTGAGAAGGGCCTGGCAGAGGACATA
  GAGAATGAGGTGGTCCAGATTACGTGGAACAGGAAGAAGCTGGGAGAGTTCTTCCAGACCAAGTACGAT
  TGGGATCTGCTGGCTGCCCGTTCCATCTGGGCTTTTGGCCCTGATGCGACTGGCCCCAACATTCTGGTG
  GATGATACTCTGCCCTCTGAGGTGGACAAGGCTCTTCTTGGTTCAGTGAAGGACAGCATCGTTCAAGGT
  TTCCAGTGGGGAACCAGGGAGGGCCCCCTCTGTGATGAATTGATTCGGAATGTCAAGTTTAAGATCCTG
  GATGCGGTGGTTGCCCAGGAGCCCCTGCACCGGGGCGGGGGCCAGATCATCCCCACAGCCAGGAGAGTC
  GTCTACTCTGCCTTCCTCATGGCTACTCCTCGTCTGATGGAGCCTTACTACTTTGTAGAGGTCCAGGCC
  CCTGCAGATTGCGTCTCTGCAGTTTATACCGTCCTGGCCAGGCGCAGGGGGCACGTGACTCAGGATGCA
  CCCATCCCAGGCTCCCCTCTGTACACCATCAAAGCTTTTATCCCGGCCATCGACTCTTTTGGCTTTGAG
  ACTGATCTCCGGACTCACACCCAGGGACAAGCCTTTTCTCTGTCTGTCTTCCACCACTGGCAGATTGTG
  CCTGGTGATCCCCTGGACAAGAGCATTGTCATCCGCCCCTTGGAGCCACAGCCAGCTCCTCACCTGGCC
  CGGGAATTCATGATCAAAACCCGCCGTAGGAAGGGCCTCAGTGAAGATGTGAGCATCAGCAAATTCTTC
  GATGATCCTATGTTGCTGGAACTTGCCAAACAGGATGTTGTGCTCAATTACCCCATGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001258354
• Insert Size: 2889 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258354.1
• RefSeq Size: 4137 bp
• RefSeq ORF: 2889 bp
• Locus ID: 9343
• UniProt ID: Q15029
• Cytogenetics: 17q21.31
• Protein Pathways: Spliceosome
• MW: 108.3 kDa
• Gene Summary: This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting protein (isoform c) is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.