CARD14 (NM_001257970) Human Untagged Clone

CAT#: SC332551

CARD14 (untagged) - Homo sapiens caspase recruitment domain family, member 14 (CARD14), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CARD14
• Synonyms: BIMP2; CARMA2; PRP; PSORS2; PSS1
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332551 representing NM_001257970.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGGGGAACTGTGCCGCAGGGACTCCGCACTCACGGCACTGGACGAGGAGACACTGTGGGAGATGATG
  GAGAGCCACCGCCACAGGATCGTACGCTGCATCTGCCCCAGCCGCCTCACCCCCTACCTGCGCCAGGCC
  AAGGTGCTGTGCCAGCTGGACGAGGAGGAGGTGCTGCACAGCCCCCGGCTCACCAACAGCGCCATGCGG
  GCCGGGCACTTGCTGGATTTGCTGAAGACTCGAGGGAAGAACGGGGCCATCGCCTTCCTGGAGAGCCTG
  AAGTTCCACAACCCTGACGTCTACACCCTGGTCACCGGGCTGCAGCCTGATGTTGACTTCAGTAACTTT
  AGCGGTCTCATGGAGACATCCAAGCTGACCGAGTGCCTGGCTGGGGCCATCGGCAGCCTGCAGGAGGAG
  CTGAACCAGGAAAAGGGGCAGAAGGAGGTGCTGCTGCGGCGGTGCCAGCAGCTGCAGGAGCACCTGGGC
  CTGGCCGAGACCCGTGCCGAGGGCCTGCACCAGCTGGAGGCTGACCACAGCCGCATGAAGCGTGAGGTT
  AGCGCACACTTCCATGAGGTGCTGAGGCTGAAGGACGAGATGCTCAGCCTCTCGCTGCACTATAGCAAT
  GCGCTGCAGGAGAAGGAGCTGGCCGCCTCACGCTGCCGCAGCCTGCAGGAGGAGCTGTATCTACTGAAG
  CAGGAGCTGCAGCGAGCCAACATGGTTTCCTCCTGTGAGCTGGAATTGCAAGAGCAGTCCCTGAGGACA
  GCCAGCGACCAGGAGTCCGGGGATGAGGAGCTGAACCGCCTGAAGGAGGAGAATGAGAAACTGCGCTCG
  CTGACTTTCAGCCTGGCGGAGAAGGACATTCTGGAGCAGAGCCTGGACGAGGCGCGGGGGAGCCGACAG
  GAGCTGGTGGAGCGCATCCACTCGCTGCGGGAGCGGGCCGTGGCTGCCGAGAGGCAGCGAGAGCAGTAC
  TGGGAAGAGAAGGAACAGACCCTGCTGCAGTTCCAGAAGAGTAAGATGGCCTGCCAACTCTACAGGGAG
  AAGGTGAATGCGCTGCAGGCCCAGGTGTGCGAGCTGCAGAAGGAGCGAGACCAGGCGTACTCCGCGAGG
  GACAGTGCTCAGAGGGAGATTTCCCAGAGCCTGGTGGAGAAGGACTCCCTCCGCAGGCAGGTGTTCGAG
  CTGACGGACCAGGTCTGCGAGCTGCGCACACAGCTTCGCCAGCTGCAGGCAGAGCCTCCGGGTGTGCTC
  AAGCAGGAAGCCAGGACCAGGGAGCCCTGTCCACGGGAGAAGCAGCGGCTGGTGCGGATGCATGCCATC
  TGCCCCAGAGACGACAGCGACTGCAGCCTCGTCAGCTCCACAGAGTCTCAGCTCTTGTCGGACCTGAGT
  GCCACGTCCAGCCGCGAGCTGGTGGACAGCTTCCGCTCCAGCAGCCCCGCGCCCCCCAGCCAGCAGTCC
  CTGTACAAGCGGGTGGCCGAGGACTTCGGGGAAGAACCCTGGTCTTTCAGCAGCTGCCTGGAGATCCCG
  GAGGGAGACCCGGGAGCCCTGCCGGGAGCTAAGGCAGGCGACCCACACCTGGATTATGAGCTCCTAGAC
  ACGGCAGACCTTCCGCAGCTGGAAAGCAGCCTGCAGCCAGTCTCCCCTGGAAGGCTTGATGTCTCGGAG
  AGCGGCGTCCTCATGCGGCGGAGGCCAGCCCGCAGGATCCTGAGCCAGGTCACCATGCTGGCGTTCCAG
  GGGGATGCATTGCTGGAGCAGATCAGCGTCATCGGCGGGAACCTCACGGGCATCTTCATCCACCGGGTC
  ACCCCGGGCTCGGCGGCGGACCAGATGGCCTTGCGCCCGGGCACCCAGATTGTGATGGTTGATTACGAA
  GCCTCAGAGCCCTTGTTCAAGGCAGTCCTGGAGGACACGACCCTGGAGGAGGCCGTGGGGCTTCTCAGG
  AGGGTGGACGGCTTCTGCTGCCTGTCTGTGAAGGTCAACACGGACGGTTATAAGAGGCTACTCCAGGAC
  CTGGAGGCCAAAGTGGCGACCTCGGGGGACTCATTCTACATCCGGGTCAACCTGGCCATGGAGGGCAGG
  GCCAAAGGGGAGCTGCAGGTGCATTGCAACGAGGTCCTGCACGTCACCGACACCATGTTCCAGGGCTGC
  GGCTGCTGGCATGCCCACCGCGTGAACTCTTACACCATGAAGGATACTGCCGCGCACGGCACCATCCCC
  AACTACTCCAGGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001257970
• Insert Size: 2223 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001257970.1
• RefSeq Size: 2612 bp
• RefSeq ORF: 2223 bp
• Locus ID: 79092
• UniProt ID: Q9BXL6
• Cytogenetics: 17q25.3
• Protein Families: Druggable Genome
• MW: 83.7 kDa
• Gene Summary: This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1. The encoded isoform 3 has a shorter C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.