GUCY1A2 (NM_001256424) Human Untagged Clone

CAT#: SC332380

GUCY1A2 (untagged) - Homo sapiens guanylate cyclase 1, soluble, alpha 2 (GUCY1A2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GUCY1A2
• Synonyms: GC-SA2; GUC1A2
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332380 representing NM_001256424.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTCTCGAAGGAAGATTTCGTCCGAGTCCTTCAGCTCCCTGGGCTCCGACTACCTGGAGACCAGCCCG
  GAGGAGGAGGGGGAGTGCCCCCTGTCTAGGCTCTGCTGGAATGGCAGCCGGAGCCCGCCCGGGCCGCTG
  GAGCCCAGCCCGGCCGCAGCTGCCGCTGCCGCCGCCCCGGCCCCGACCCCGGCTGCTTCTGCCGCCGCC
  GCCGCTGCCACTGCCGGGGCCAGGAGGGTGCAGCGCCGGAGGCGGGTCAACCTGGACTCGCTGGGCGAG
  AGCATCAGCCGCCTGACGGCGCCCTCGCCTCAGACGATACAGCAGACTCTCAAGAGGACACTGCAGTAT
  TATGAACATCAAGTTATTGGTTACAGGGATGCAGAAAAGAATTTCCACAATATCTCTAACAGATGCTCC
  TATGCAGACCACTCCAACAAAGAAGAAATTGAAGATGTCTCAGGAATTCTTCAGTGTACTGCTAATATA
  CTCGGTTTGAAGTTTGAGGAAATTCAAAAAAGATTTGGTGAAGAGTTCTTTAATATATGCTTTCATGAG
  AATGAGAGAGTCCTTCGAGCTGTAGGTGGCACTTTGCAGGACTTTTTTAACGGCTTTGATGCTTTGTTG
  GAACACATTAGAACTTCTTTTGGAAAACAGGCCACTCTGGAGTCACCATCTTTCCTATGCAAAGAGCTC
  CCTGAAGGTACTCTCATGCTCCACTACTTCCACCCTCACCATATTGTGGGGTTTGCAATGCTGGGGATG
  ATTAAGGCTGCAGGAAAGAAGATCTATCGGCTGGATGTGGAAGTGGAACAGGTTGCAAATGAGAAGCTA
  TGCTCTGATGTTTCAAACCCAGGCAATTGTAGCTGTCTTACTTTCCTTATCAAAGAATGTGAAAATACT
  AATATCATGAAGAACCTTCCACAGGGAACCTCCCAAGTTCCTGCGGACCTCAGAATTAGCATCAACACC
  TTCTGTAGAGCCTTCCCTTTCCACTTGATGTTTGATCCCAGCATGTCAGTCCTTCAGTTGGGGGAAGGT
  CTAAGGAAGCAGCTTCGATGTGACACTCACAAAGTGCTCAAGTTTGAGGACTGCTTCGAGATTGTATCT
  CCAAAGGTTAATGCCACCTTTGAAAGGGTCCTGCTGCGACTGTCTACCCCGTTTGTGATTAGAACCAAG
  CCTGAGGCTTCTGGCTCTGAAAATAAAGACAAGGTGATGGAAGTCAAAGGACAAATGATCCATGTTCCA
  GAATCAAATTCCATTTTATTTTTGGGCTCTCCATGTGTGGACAAGTTGGATGAACTCATGGGCCGAGGG
  CTACATCTCTCAGACATCCCTATCCATGATGCCACCCGAGATGTCATTTTGGTTGGTGAGCAGGCAAAG
  GCCCAAGATGGGTTGAAGAAAAGGATGGATAAATTAAAGGCAACTTTAGAAAGAACTCACCAGGCCCTG
  GAAGAAGAGAAAAAGAAGACAGTGGATCTTCTATATTCTATTTTCCCTGGTGATGTAGCCCAGCAATTA
  TGGCAAGGGCAGCAAGTACAGGCCAGAAAGTTTGATGATGTCACCATGCTCTTTTCAGACATTGTTGGC
  TTCACAGCCATATGTGCCCAGTGTACTCCCATGCAAGTAATCAGCATGCTGAATGAACTGTACACCAGA
  TTTGACCACCAGTGTGGATTTTTGGATATTTATAAGGTGGAAACAATAGGTGATGCCTACTGTGTTGCA
  GCAGGGCTCCACAGAAAAAGCCTCTGCCATGCTAAACCCATTGCTCTGATGGCCTTGAAGATGATGGAA
  CTTTCAGAAGAGGTGCTGACACCTGATGGAAGACCGATTCAGCCCCAGAGGAGTGAACTCCTTTTCTCC
  TTTCCTGTTTCCATCCAGCTGGTTCCAGATCAGCATCAAAGTGAAACGGATTTGGGTACTGAAAAGATG
  AGGATAGGAATTCACTCAGGCTCCGTGCTGGCTGGAGTTGTTGGGGTGCGAATGCCACGTTATTGCCTG
  TTTGGAAATAATGTCACACTGGCAAGCAAATTCGAGTCGGGAAGTCACCCTCGGCGCATCAATGTCAGC
  CCAACCACTTACCAATTATTAAAACGAGAAGAAAGTTTCACATTCATTCCGCGGTCTCGTGAAGAGCTT
  CCAGACAACTTTCCAAAGGAAATTCCTGGGATCTGCTATTTCCTGGAGGTAAGGACTGGTCCAAAGCCA
  CCAAAGCCTTCTCTTTCTTCGTCGAGAATAAAAAAGGTTTCCTACAACATCGGCACCATGTTCCTCCGG
  GAGACAAGCCTCTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256424
• Insert Size: 2292 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256424.1
• RefSeq Size: 16233 bp
• RefSeq ORF: 2292 bp
• Locus ID: 2977
• UniProt ID: P33402
• Cytogenetics: 11q22.3
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• MW: 85.3 kDa
• Gene Summary: Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.