GUCY1A2 (NM_001256424) Human Tagged ORF Clone

CAT#: RG234852

GUCY1A2 (tGFP-tagged) - Homo sapiens guanylate cyclase 1, soluble, alpha 2 (GUCY1A2), transcript variant 1

Product Images

Circular map for RG234852

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GUCY1A2
• Synonyms: GC-SA2; GUC1A2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234852 representing NM_001256424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGAAGGAAGATTTCGTCCGAGTCCTTCAGCTCCCTGGGCTCCGACTACCTGGAGACCAGCCCGG
  AGGAGGAGGGGGAGTGCCCCCTGTCTAGGCTCTGCTGGAATGGCAGCCGGAGCCCGCCCGGGCCGCTGGA
  GCCCAGCCCGGCCGCAGCTGCCGCTGCCGCCGCCCCGGCCCCGACCCCGGCTGCTTCTGCCGCCGCCGCC
  GCTGCCACTGCCGGGGCCAGGAGGGTGCAGCGCCGGAGGCGGGTCAACCTGGACTCGCTGGGCGAGAGCA
  TCAGCCGCCTGACGGCGCCCTCGCCTCAGACGATACAGCAGACTCTCAAGAGGACACTGCAGTATTATGA
  ACATCAAGTTATTGGTTACAGGGATGCAGAAAAGAATTTCCACAATATCTCTAACAGATGCTCCTATGCA
  GACCACTCCAACAAAGAAGAAATTGAAGATGTCTCAGGAATTCTTCAGTGTACTGCTAATATACTCGGTT
  TGAAGTTTGAGGAAATTCAAAAAAGATTTGGTGAAGAGTTCTTTAATATATGCTTTCATGAGAATGAGAG
  AGTCCTTCGAGCTGTAGGTGGCACTTTGCAGGACTTTTTTAACGGCTTTGATGCTTTGTTGGAACACATT
  AGAACTTCTTTTGGAAAACAGGCCACTCTGGAGTCACCATCTTTCCTATGCAAAGAGCTCCCTGAAGGTA
  CTCTCATGCTCCACTACTTCCACCCTCACCATATTGTGGGGTTTGCAATGCTGGGGATGATTAAGGCTGC
  AGGAAAGAAGATCTATCGGCTGGATGTGGAAGTGGAACAGGTTGCAAATGAGAAGCTATGCTCTGATGTT
  TCAAACCCAGGCAATTGTAGCTGTCTTACTTTCCTTATCAAAGAATGTGAAAATACTAATATCATGAAGA
  ACCTTCCACAGGGAACCTCCCAAGTTCCTGCGGACCTCAGAATTAGCATCAACACCTTCTGTAGAGCCTT
  CCCTTTCCACTTGATGTTTGATCCCAGCATGTCAGTCCTTCAGTTGGGGGAAGGTCTAAGGAAGCAGCTT
  CGATGTGACACTCACAAAGTGCTCAAGTTTGAGGACTGCTTCGAGATTGTATCTCCAAAGGTTAATGCCA
  CCTTTGAAAGGGTCCTGCTGCGACTGTCTACCCCGTTTGTGATTAGAACCAAGCCTGAGGCTTCTGGCTC
  TGAAAATAAAGACAAGGTGATGGAAGTCAAAGGACAAATGATCCATGTTCCAGAATCAAATTCCATTTTA
  TTTTTGGGCTCTCCATGTGTGGACAAGTTGGATGAACTCATGGGCCGAGGGCTACATCTCTCAGACATCC
  CTATCCATGATGCCACCCGAGATGTCATTTTGGTTGGTGAGCAGGCAAAGGCCCAAGATGGGTTGAAGAA
  AAGGATGGATAAATTAAAGGCAACTTTAGAAAGAACTCACCAGGCCCTGGAAGAAGAGAAAAAGAAGACA
  GTGGATCTTCTATATTCTATTTTCCCTGGTGATGTAGCCCAGCAATTATGGCAAGGGCAGCAAGTACAGG
  CCAGAAAGTTTGATGATGTCACCATGCTCTTTTCAGACATTGTTGGCTTCACAGCCATATGTGCCCAGTG
  TACTCCCATGCAAGTAATCAGCATGCTGAATGAACTGTACACCAGATTTGACCACCAGTGTGGATTTTTG
  GATATTTATAAGGTGGAAACAATAGGTGATGCCTACTGTGTTGCAGCAGGGCTCCACAGAAAAAGCCTCT
  GCCATGCTAAACCCATTGCTCTGATGGCCTTGAAGATGATGGAACTTTCAGAAGAGGTGCTGACACCTGA
  TGGAAGACCGATTCAGCCCCAGAGGAGTGAACTCCTTTTCTCCTTTCCTGTTTCCATCCAGCTGGTTCCA
  GATCAGCATCAAAGTGAAACGGATTTGGGTACTGAAAAGATGAGGATAGGAATTCACTCAGGCTCCGTGC
  TGGCTGGAGTTGTTGGGGTGCGAATGCCACGTTATTGCCTGTTTGGAAATAATGTCACACTGGCAAGCAA
  ATTCGAGTCGGGAAGTCACCCTCGGCGCATCAATGTCAGCCCAACCACTTACCAATTATTAAAACGAGAA
  GAAAGTTTCACATTCATTCCGCGGTCTCGTGAAGAGCTTCCAGACAACTTTCCAAAGGAAATTCCTGGGA
  TCTGCTATTTCCTGGAGGTAAGGACTGGTCCAAAGCCACCAAAGCCTTCTCTTTCTTCGTCGAGAATAAA
  AAAGGTTTCCTACAACATCGGCACCATGTTCCTCCGGGAGACAAGCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234852 representing NM_001256424
  Red=Cloning site Green=Tags(s)
  
  MSRRKISSESFSSLGSDYLETSPEEEGECPLSRLCWNGSRSPPGPLEPSPAAAAAAAAPAPTPAASAAAA
  AATAGARRVQRRRRVNLDSLGESISRLTAPSPQTIQQTLKRTLQYYEHQVIGYRDAEKNFHNISNRCSYA
  DHSNKEEIEDVSGILQCTANILGLKFEEIQKRFGEEFFNICFHENERVLRAVGGTLQDFFNGFDALLEHI
  RTSFGKQATLESPSFLCKELPEGTLMLHYFHPHHIVGFAMLGMIKAAGKKIYRLDVEVEQVANEKLCSDV
  SNPGNCSCLTFLIKECENTNIMKNLPQGTSQVPADLRISINTFCRAFPFHLMFDPSMSVLQLGEGLRKQL
  RCDTHKVLKFEDCFEIVSPKVNATFERVLLRLSTPFVIRTKPEASGSENKDKVMEVKGQMIHVPESNSIL
  FLGSPCVDKLDELMGRGLHLSDIPIHDATRDVILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKT
  VDLLYSIFPGDVAQQLWQGQQVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGFL
  DIYKVETIGDAYCVAAGLHRKSLCHAKPIALMALKMMELSEEVLTPDGRPIQPQRSELLFSFPVSIQLVP
  DQHQSETDLGTEKMRIGIHSGSVLAGVVGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKRE
  ESFTFIPRSREELPDNFPKEIPGICYFLEVRTGPKPPKPSLSSSRIKKVSYNIGTMFLRETSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256424
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256424.2
• RefSeq Size: 16233 bp
• RefSeq ORF: 2292 bp
• Locus ID: 2977
• UniProt ID: P33402
• Cytogenetics: 11q22.3
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• Gene Summary: Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]