PIWIL3 (NM_001255975) Human Untagged Clone

CAT#: SC332274

PIWIL3 (untagged) - Homo sapiens piwi-like RNA-mediated gene silencing 3 (PIWIL3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIWIL3
• Synonyms: HIWI3
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332274 representing NM_001255975.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGCCTGGTAGGGCAAGGACTCGCGCCCGAGGCAGAGCCCGCCGCAGGGAGAGCTACCAACAAGAGGCA
  CCTGGGGGACCCAGAGCACCTGGATCAGCTACAACCCAGGAGCCCCCTCAGTTGCAGTCGACACCCCGG
  CCGCTGCAGGAGGAAGTCCCAGTGGTTAGACCTCTGCAGCCAAGAGCAGCAAGAGGAGGAGCAGGAGGA
  GGAGCACAGTCTCAAGGGGTGAAGGAACCTGGACCTGAGGCTGGGTTGCATACAGCGCCCTTGCAGGAG
  AGAAGGATTGGTGGAGTTTTTCAAGACCTGGTGGTGAACACCAGGCAAGATATGAAGCATGTTAAAGAC
  TCAAAAACAGGTTCAGAGGGTACAGTGGTACAGCTACTCGCCAACCACTTCCGAGTGATATCTCGTCCT
  CAGTGGGTTGCATATAAATACAACGTTGACTACAAACCAGACATAGAAGATGGAAATCTCCGTACAATT
  TTACTTGATCAACATAGAAGGAAATTTGGAGAGCGCCATATATTTGATGGAAACTCTTTATTATTATCT
  CGGCCACTAAAAGAGCGGAGAGTGGAATGGTTGAGCACAACCAAAGACAAAAACATCGTGAAGATTACA
  GTTGAGTTTTCCAAAGAACTCACGCCCACGTCGCCAGATTGCCTACGCTATTACAACATTCTCTTTAGA
  AGAACTTTCAAGCTGCTGGATTTTGAACAAGTTGGTCGCAACTATTATACCAAAAAGAAGGCCATTCAG
  TTATACCGTCATGGTACCAGTTTGGAAATCTGGCTTGGTTATGTTACTTCTGTTCTTCAATACGAAAAC
  AGCATTACCCTCTGTGCCGATGTGAGCCACAAACTGCTCCGAATAGAAACTGCTTATGATTTCATAAAG
  AGAACATCTGCCCAGGCCCAGACAGGAAACATCCGAGAGGAAGTAACTAATAAATTAATTGGATCAATT
  GTTCTGACAAAATACAACAACAAAACCTACAGAGTAGATGATATTGATTGGAAGCAGAATCCTGAAGAC
  ACATTTAACAAATCAGATGGCAGCAAAATCACCTATATAGACTACTACAGGCAGCAACATAAAGAAATT
  GTCACAGTGAAGAAACAGCCACTTTTGGTCAGCCAGGGCAGATGGAAAAAGGGCCTAACGGGTACACAA
  CGTGAACCTATCCTGCTGATTCCTCAGCTGTGCCACATGACAGGTCTAACAGATGAAATATGTAAAGAT
  TATAGCATTGTGAAAGAATTGGCTAAACATACAAGATTGAGTCCAAGAAGAAGGCATCATACATTAAAA
  GAATTCATCAATACTCTACAAGATAATAAAAAAGTACGAGAGTTACTTCAACTCTGGGATTTGAAATTT
  GATACCAATTTTTTGTCCGTCCCGGGAAGAGTTTTGAAAAACGCAAACATCGTGCAAGGCAGAAGAATG
  GTTAAAGCCAATTCACAAGGAGACTGGTCAAGAGAAATAAGAGAATTACCCTTACTTAATGCAATGCCA
  CTACATAGTTGGCTCATACTCTATAGCAGGAGCAGTCACAGAGAAGCCATGTCCTTAAAGGGTCATCTA
  CAGAGTGTCACAGCCCCCATGGGCATAACTATGAAACCAGCAGAAATGATTGAAGTAGATGGTGATGCT
  AACTCCTATATAGACACATTACGGAAATATACTAGACCAACACTGCAGATGGTGATTTGTATCCTGCCC
  AATGATGACAAACGTAGATATGACAGCATAAAAAGATACCTATGTACCAAATGCCCAATTCCAAGCCAG
  TGTGTGGTGAAAAAGACCTTAGAAAAAGTCCAGGCAAGGACCATCGTCACCAAGATTGCCCAGCAGATG
  AATTGCAAGATGGGAGGAGCCCTCTGGAAGGTGGAGACAGACGTACAAAGAACAATGTTCGTTGGCATT
  GATTGTTTCCACGATATCGTAAATCGACAGAAATCAATAGCAGGATTTGTTGCAAGTACCAATGCTGAA
  TTAACAAAGTGGTACTCTCAATGTGTCATCCAGAAAACAGGAGAAGAGCTTGTGAAAGAGCTGGAGATC
  TGCTTGAAAGCTGCCCTGGATGTCTGGTGTAAAAACGAATCATCGATGCCACATTCTGTTATTGTGTAT
  CGGGATGGAGTGGGAGATGGTCAGCTTCAAGCATTGCTTGACCATGAAGCGAAAAAGATGTCGACCTAC
  TTAAAAACCATCTCTCCTAACAATTTCACTCTAGCTTTCATTGTGGTGAAGAAACGAATAAACACTAGA
  TTTTTTCTTAAACATGGAAGCAATTTTCAAAATCCACCTCCAGGAACAGTTATTGATGTAGAGTTGACT
  AGGAATGAATGGTATGACTTTTTTATTGTGAGTCAGTCTGTGCAAGATGGGACTGTTACCCCCACTCAT
  TATAACGTCATCTATGACACGATTGGCTTGAGCCCAGATACAGTACAGCGTTTAACATATTGTCTATGC
  CACATGTATTATAATTTGCCAGGCATCATCCGAGTTCCAGCGCCTTGCCACTATGCCCACAAGCTGGCT
  TACCTCGTGGGGCAGTCCATTCACCAGGAACCGAATCGTTCCTTGTCAACTCGTCTCTTTTACCTTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001255975
• Insert Size: 2622 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001255975.1
• RefSeq Size: 3481 bp
• RefSeq ORF: 2622 bp
• Locus ID: 440822
• Cytogenetics: 22q11.23
• Protein Pathways: Dorso-ventral axis formation
• MW: 100.1 kDa
• Gene Summary: This gene encodes a member of the PIWI subfamily of Argonaute family proteins. This subfamily of proteins contains a PAZ domain, found in proteins involved in RNA-mediated gene silencing, and a C-terminal Piwi domain. The encoded protein is thought to function in maintenance of germline cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (2) uses an alternate splice site compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.