RGNEF (ARHGEF28) (NM_001244364) Human Untagged Clone

CAT#: SC332072

ARHGEF28 (untagged) - Homo sapiens Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RGNEF
• Synonyms: p190RHOGEF; RGNEF; RIP2
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332072 representing NM_001244364.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGATTCTGACTCCGACTCACCTTTTAACTACTCGTGGCCTTCCTTTCCCAAAATGAAGATTCGAAGA
  AGGACGTCCAAACAAGATCGCTCCTTCGATATCCTAAAAAAATCCAAGCCGCCCTCGACATTGCTTGCT
  GCAGGCCGGCTTTCAGACATGCTGAATGGAGGTGATGAAGTCTACGCTAACTGTATGGTGATTGATCAG
  GTTGGTGATTTGGATATCAGCTATATTAATATAGAGGGAATCACTGCCACTACCAGCCCTGAATCCAGA
  GGTTGCACTCTGTGGCCTCAGAGCAGCAAACACACCCTTCCTACAGAAACCAGTCCCAGTGTGTACCCA
  CTTAGTGAAAATGTCGAAGGGACAGCACACACTGAAGCCCAGCAGTCCTTCATGTCACCATCAAGTTCG
  TGTGCTTCCAACTTGAATCTTTCTTTTGGTTGGCATGGATTTGAAAAGGAACAAAGTCATCTAAAGAAA
  AGAAGTTCTAGCCTTGATGCCTTGGACGCCGACAGTGAAGGGGAAGGGCATTCTGAGCCATCCCACATC
  TGTTACACTCCAGGGTCTCAGAGCTCCTCAAGAACTGGGATTCCTAGTGGGGATGAATTGGACTCTTTT
  GAGACTAACACTGAACCGGATTTTAATATCTCCAGGGCTGAATCCCTTCCTCTATCAAGTAATCTACAG
  TCGAAGGAATCACTGCTTTCTGGAGTTCGCTCACGTTCTTATTCTTGCTCATCACCCAAAATTTCTTTA
  GGAAAAACTCGTTTGGTGCGTGAATTAACAGTATGCAGTTCAAGTGAAGAGCAAAGAGCTTACAGCTTA
  TCGGAGCCACCAAGAGAAAACAGAATTCAGGAAGAAGAATGGGATAAATACATCATACCTGCCAAATCA
  GAGTCTGAAAAATATAAAGTGAGTCGAACTTTCAGTTTCCTCATGAATAGGATGACTAGCCCTCGGAAT
  AAATCAAAGACAAAAAGCAAGGATGCCAAAGATAAAGAGAAGCTGAATCGACATCAGTTTGCCCCAGGA
  ACATTCTCTGGGGTTCTGCAGTGTTTGGTTTGTGATAAAACACTCCTGGGGAAAGAGTCACTGCAGTGT
  TCTAACTGTAATGCAAATGTGCACAAAGGTTGTAAAGATGCTGCGCCTGCATGCACCAAGAAATTCCAA
  GAGAAATATAACAAGAACAAACCACAGACCATCCTTGGAAATTCTTCATTTAGAGACATCCCACAGCCT
  GGTCTCTCCTTGCACCCTTCTTCCTCCGTGCCTGTTGGATTGCCGACTGGAAGGAGGGAGACTGTGGGA
  CAGGTCCATCCATTGTCCAGAAGTGTTCCAGGCACCACCTTGGAAAGCTTCAGGAGGTCAGCCACATCC
  TTGGAGTCTGAGAGTGACCATAACAGCTGCAGAAGCAGGTCTCATTCTGATGAGCTGCTACAGTCCATG
  GGCTCTTCTCCCTCTACAGAGTCTTTCATAATGGAAGATGTTGTGGATTCTTCTCTGTGGAGTGACCTC
  AGCAGTGATGCCCAGGAGTTTGAAGCAGAATCTTGGAGTCTTGTGGTGGATCCCTCATTTTGTAATAGG
  CAGGAGAAGGATGTCATCAAAAGACAGGATGTCATTTTTGAGCTAATGCAAACAGAGATGCATCACATC
  CAGACCCTGTTCATCATGTCTGAGATCTTCAGGAAAGGCATGAAAGAGGAGCTGCAGCTGGACCACAGC
  ACCGTGGATAAAATTTTCCCCTGTTTAGATGAGTTGCTTGAAATCCACAGGCATTTCTTCTACAGTATG
  AAGGAACGAAGGCAGGAATCCTGTGCTGGCAGCGACAGGAATTTTGTGATCGACCGAATTGGAGATATT
  TTGGTACAACAGTTTTCAGAAGAAAATGCAAGTAAAATGAAGAAAATATATGGAGAATTCTGTTGCCAT
  CATAAAGAAGCTGTTAACCTCTTTAAAGAACTCCAGCAGAATAAAAAGTTTCAGAATTTTATTAAGCTC
  CGAAATAGTAATCTTTTGGCTCGACGCCGAGGAATTCCAGAATGCATTCTGTTGGTCACTCAGCGTATT
  ACAAAATACCCTGTCTTGGTGGAAAGGATATTGCAGTACACAAAGGAAAGAACTGAGGAACATAAAGAC
  TTACGCAAAGCGCTTTGCTTAATTAAAGACATGATTGCAACAGTGGATTTAAAAGTCAATGAATATGAG
  AAAAACCAAAAATGGCTTGAGATCCTAAATAAGATTGAAAACAAAACATACACGAAGCTCAAAAATGGA
  CATGTGTTTAGGAAGCAGGCACTGATGAGTGAAGAAAGGACTCTGTTATATGATGGCCTTGTTTACTGG
  AAAACTGCTACAGGTCGTTTCAAAGATATCCTAGCTCTACTTCTAACTGATGTGCTGCTCTTTTTACAA
  GAAAAAGACCAGAAATACATCTTTGCAGCCGTTGATCAGAAGCCATCAGTTATTTCCCTTCAAAAGCTT
  ATTGCTAGAGAAGTTGCTAATGAGGAGAGAGGAATGTTTCTGATCAGTGCTTCATCTGCTGGTCCTGAG
  ATGTATGAAATTCACACCAATTCCAAGGAGGAACGCAATAACTGGATGAGACGGATCCAGCAGGCTGTA
  GAAAGTTGTCCTGAAGAAAAAGGGGGAAGGACAAGTGAATCTGATGAAGACAAGAGGAAAGCTGAAGCC
  AGAGTGGCCAAAATTCAGCAATGTCAAGAAATACTCACTAACCAAGACCAACAAATTTGTGCGTATTTG
  GAGGAGAAGCTGCATATCTATGCTGAACTTGGAGAACTGAGCGGATTTGAGGACGTCCATCTAGAGCCC
  CACCTCCTTATTAAACCTGACCCAGGCGAGCCTCCCCAGGCAGCCTCATTACTGGCAGCAGCACTGAAA
  GAAGCTGAGAGCCTACAAGTTGCAGTGAAGGCCTCACAGATGGGCGCCGTGAGTCAATCATGTGAGGAC
  AGTTGTGGAGACTCTGTCTTGGCGGACACACTCAGTTCTCATGATGTACCAGGATCACCGACTGCCTCA
  TTAGTCACAGGAGGGAGAGAAGGAAGAGGCTGTTCGGATGTGGATCCCGGGATCCAGGGTGTGGTAACC
  GACTTGGCCGTCTCTGATGCAGGGGAGAAGGTGGAATGTAGAAATTTTCCAGGTTCTTCACAATCAGAG
  ATTATACAAGCCATACAGAATTTAACCCGTCTCTTATACAGCCTTCAGGCCGCCTTGACCATTCAGGAC
  AGCCACATTGAGATCCACAGGCTGGTTCTCCAGCAGCAGGAGGGCCTGTCTCTCGGCCACTCTATCCTC
  CGAGGCGGCCCCTTGCAGGACCAGAAGTCTCGCGACGCGGACAGGCAGCATGAGGAGCTGGCCAATGTG
  CACCAGCTTCAGCACCAGCTCCAGCAGGAGCAGCGGCGCTGGCTGCGCAGGTGTGAGCAGCAGCAGCGG
  GCGCAGGCGACCAGGGAGAGCTGGCTGCAGGAGCGGGAGCGGGAGTGCCAGTCGCAGGAGGAGCTGCTG
  CTGCGGAGCCGGGGCGAGCTGGACCTCCAGCTCCAGGAGTACCAGCACAGCCTGGAGCGGCTGAGGGAG
  GGCCAGCGCCTGGTGGAGAGGGAGCAGGCGAGGATGCGGGCCCAGCAGAGCCTGCTGGGCCACTGGAAG
  CACGGCCGGCAGAGGAGCCTGCCCGCGGTGCTCCTTCCGGGTGGCCCCGAGGTAATGGAACTTAATCGA
  TCTGAGAGTTTATGTCATGAAAACTCATTCTTCATCAATGAAGCTTTAGTACAAATGTCATTTAACACT
  TTCAACAAACTGAATCCATCAGTTATCCATCAGGATGCCACTTACCCTACAACTCAATCTCATTCTGAC
  TTGGTGAGGACTAGTGAACATCAAGTAGACCTCAAGGTGGACCCTTCTCAGCCTTCGAATGTCAGTCAC
  AAACTGTGGACAGCCGCTGGTTCCGGCCATCAGATACTTCCTTTCCATGAAAGCAGCAAGGATTCTTGT
  AAAAATGATTTGGACACCTCCCACACTGAGTCCCCAACCCCCCATGACTCAAATTCACACCGCCCTCAA
  CTGCAGGCGTTTATAACAGAAGCAAAGCTAAATCTACCGACAAGGACAATGACCAGACAAGATGGGGAA
  ACTGGAGATGGAGCCAAAGAAAATATTGTTTACCTCTAA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001244364
• Insert Size: 4179 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244364.1
• RefSeq Size: 5247 bp
• RefSeq ORF: 4179 bp
• Locus ID: 64283
• UniProt ID: Q8N1W1
• Cytogenetics: 5q13.2
• MW: 157.3 kDa
• Gene Summary: This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform 3) is shorter and has a distinct N-terminus, compared to isoform 1.