RUNX1T1 (NM_001198625) Human Untagged Clone

CAT#: SC331210

RUNX1T1 (untagged) - Homo sapiens runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RUNX1T1
• Synonyms: AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331210 representing NM_001198625.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGCCTGATCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAGACGCAATCTAGG
  CTGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAGAACCAGTTCATTTACACCG
  ACAACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGCCCCCTCACCACCCAATGGC
  TTCAGCAATGGGCCTTCCTCTTCTTCCTCCTCCTCTCTGGCTAATCAACAGCTGCCCCCAGCCTGTGGT
  GCCAGGCAACTCAGCAAGCTGAAAAGGTTCCTTACTACCCTGCAGCAGTTTGGCAATGACATTTCACCC
  GAGATAGGAGAAAGAGTTCGCACCCTCGTTCTGGGACTAGTGAACTCCACTTTGACAATTGAAGAATTT
  CATTCCAAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCATTTTTGAAGGCCAAC
  TTGCCCCTGCTGCAGCGTGAGCTCCTCCACTGCGCAAGACTGGCCAAACAGAACCCTGCCCAGTACCTC
  GCCCAGCATGAACAGCTGCTTCTGGATGCCAGCACCACCTCACCTGTTGACTCCTCAGAGCTGCTTCTC
  GATGTGAACGAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTTGACAGAGAGCCT
  TTGCACTCAGAACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTACAGTCCAAATAAC
  GGCTTATCCTACCAGCCCAATGGCCTGCCTCACCCTACCCCACCTCCACCTCAGCATTACCGTTTGGAT
  GATATGGCCATTGCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGACCTCAGGGACAGA
  AACAGACCTATGGGGTTGCATGGCACACGTCAAGAAGAAATGATTGATCACAGACTAACAGACAGAGAA
  TGGGCAGAAGAGTGGAAACATCTTGACCATCTGTTAAACTGCATAATGGACATGGTAGAAAAAACAAGG
  CGATCTCTCACCGTACTAAGGCGGTGTCAAGAAGCAGACCGGGAAGAATTGAATTACTGGATCCGGCGG
  TACAGTGACGCCGAGGACTTAAAAAAAGGTGGCGGCAGTAGCAGCAGCCACTCTAGGCAGCAGAGTCCC
  GTCAACCCAGACCCAGTTGCACTAGACGCGCATCGGGAATTCCTTCACAGGCCTGCGTCTGGATACGTG
  CCAGAGGAGATCTGGAAGAAAGCTGAGGAGGCCGTCAATGAGGTGAAGCGCCAGGCGATGACGGAGCTG
  CAGAAGGCCGTGTCTGAGGCGGAGCGGAAAGCCCACGACATGATCACAACAGAGAGGGCCAAGATGGAG
  CGCACGGTCGCCGAGGCCAAACGGCAGGCGGCGGAGGACGCACTGGCAGTTATCAATCAGCAGGAGGAT
  TCAAGCGAGAGTTGCTGGAATTGTGGCCGTAAAGCGAGTGAAACCTGCAGTGGCTGTAACACAGCCCGA
  TACTGTGGCTCATTTTGCCAGCACAAAGACTGGGAGAAGCACCATCACATCTGTGGACAGACCCTGCAG
  GCCCAGCAGCAGGGAGACACACCTGCAGTCAGCTCCTCTGTCACGCCCAACAGCGGGGCTGGGAGCCCG
  ATGGACACACCACCAGCAGCCACTCCGAGGTCAACCACCCCGGGAACCCCTTCCACCATAGAGACAACC
  CCTCGCTAG
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198625
• Insert Size: 1734 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198625.1
• RefSeq Size: 7769 bp
• RefSeq ORF: 1734 bp
• Locus ID: 862
• UniProt ID: Q06455
• Cytogenetics: 8q21.3
• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• MW: 64.4 kDa
• Gene Summary: This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (5) utilizes four alternate exons in the 5' UTR compared to variant 1. Variants 1, 5 and 6 all encode isoform A, also known as MTG8a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.