ADK (NM_001202449) Human Untagged Clone

CAT#: SC329655

ADK (untagged) - Homo sapiens adenosine kinase (ADK), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADK
• Synonyms: AK
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC329655 representing NM_001202449.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGACGTCAGTCAGAGAAAATATTCTCTTTGGAATGGGAAATCCTCTGCTTGACATCTCTGCTGTAGTG
  GACAAAGATTTCCTTGATAAGTTTGATGAACTTGTGAAAAAATTCAAAGTCGAATATCATGCTGGTGGC
  TCTACCCAGAATTCAATTAAAGTGGCTCAGTGGATGATTCAACAGCCACACAAAGCAGCAACATTTTTT
  GGATGCATTGGGATAGATAAATTTGGGGAGATCCTGAAGAGAAAAGCTGCTGAAGCCCATGTGGATGCT
  CATTACTACGAGCAGAATGAGCAGCCAACAGGAACTTGTGCTGCATGCATCACTGGTGACAACAGGTCC
  CTCATAGCTAATCTTGCTGCTGCCAATTGTTATAAAAAGGAAAAACATCTTGATCTGGAGAAAAACTGG
  ATGTTGGTAGAAAAAGCAAGAGTTTGTTATATAGCAGGCTTTTTTCTTACAGTTTCCCCAGAGTCAGTA
  TTAAAGGTGGCTCACCATGCTTCTGAAAACAACAGGATTTTCACTTTGAATCTATCTGCACCGTTTATT
  AGCCAGTTCTACAAGGAATCATTGATGAAAGTTATGCCTTATGTTGATATACTTTTTGGAAATGAGACA
  GAAGCTGCCACTTTTGCTAGAGAGCAAGGCTTTGAGACTAAAGACATTAAAGAGATAGCCAAAAAGACA
  CAAGCCCTGCCAAAGATGAACTCAAAGAGGCAGCGAATCGTGATCTTCACCCAAGGGAGAGATGACACT
  ATAATGGCTACAGAAAGTGAAGTCACTGCTTTTGCTGTCTTGGATCAAGACCAGAAAGAAATTATTGAT
  ACCAATGGAGCTGGAGATGCATTTGTTGGAGGTTTTCTGTCTCAACTGGTCTCTGACAAGCCTCTGACT
  GAATGTATCCGTGCTGGCCACTATGCAGCAAGCATCATAATTAGACGGACTGGCTGCACCTTTCCTGAG
  AAGCCAGACTTCCACTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001202449
• Insert Size: 984 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202449.1
• RefSeq Size: 2234 bp
• RefSeq ORF: 984 bp
• Locus ID: 132
• UniProt ID: P55263
• Cytogenetics: 10q22.2|10q11-q24
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• MW: 36.6 kDa
• Gene Summary: This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform c), compared to isoform a.